Evidence for a direct inhibitory effect of ethanol upon gonadotropin secretion at the pituitary level.
Evidence for a direct inhibitory effect of ethanol upon gonadotropin secretion at the pituitary level.
复制标题
乙醇对垂体水平促性腺激素分泌有直接抑制作用的证据。
DOI：
10.1111/j.1530-0277.1983.tb05430.x
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
VanThiel,DH
中科院分区：
文献类型：
--
作者：
Schade,RR;Bonner,G;Gay,VC;VanThiel,DH
从成年雄性大鼠垂体中收获的混合大鼠垂体细胞在组织培养中维持长达240小时。在每次实验结束时通过台盼蓝拒染法测定的存活率大于90%。在孵育液中加入乙醇（300 mg/100 ml）前后，测定促黄体生成素（LH）向培养液中的基础分泌量。将经处理培养物的乙醇减少（p <0.01）的LH分泌与对照培养物进行比较。基于这一初步的体外研究与大鼠组织和先前的研究中进行的人和动物，我们认为，乙醇抑制LH分泌在垂体水平。
Mixed rat pituitary cells harvested from pituitaries obtained from adult male rats were maintained in tissue culture for periods up to 240 hr. Viability determined by trypan blue exclusion at the end of each experiment was greater than 90%. Basal secretory rats of luteiniring hormone (LH) into the culture medium were determined before and after the addition of ethanol (300 mg/100 ml) to the Incubation medium. Ethanol‐reduced (p <.01) LH Secretion of treated cultures were compared to control cultures. Based upon this preliminary in vitro study with rat tissue and on prior studies performed in man and animals, we suggest that ethanol inhibits LH secretion at the pituitary level.