De Novo Transcriptome Analysis of Plant Pathogenic Fungus Myrothecium roridum and Identification of Genes Associated with Trichothecene Mycotoxin Biosynthesis.
De Novo Transcriptome Analysis of Plant Pathogenic Fungus Myrothecium roridum and Identification of Genes Associated with Trichothecene Mycotoxin Biosynthesis.
复制标题
植物病原真菌红色漆斑菌的从头转录组分析及单端孢霉毒素生物合成相关基因的鉴定
DOI：
10.3390/ijms18030497
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发表时间：
2017-02-25
影响因子：
5.6
通讯作者：
Li S
中科院分区：
文献类型：
--
作者：
Ye W;Liu T;Zhu M;Zhang W;Li H;Huang Z;Li S
疣孢漆斑菌（Myrothecium roridum）是一种植物病原真菌，可侵染多种作物，导致大豆、棉花、玉米、辣椒、番茄等经济作物减产。迄今为止，对M.罗里丹仍不清楚。从M. Rhodium，并认为该菌的植物致病因子为二孢霉烯。罗里丹本研究对M.使用Illumina Hiseq 2000对不同孵育持续时间下的螺旋藻进行测序。结果表明，M.从8.0 Gb干净读段获得roridum。M.结果表明，丝裂原活化蛋白激酶信号通路在丝裂原活化蛋白激酶的致病性中也起重要作用。罗里丹并对与二萜烯生物合成相关的基因进行了注释。这些基因的表达水平也预测和验证通过定量实时聚合酶链反应。克隆并表达了编码β-亚麻酸二烯合成酶的Tri 5基因，纯化的β-亚麻酸二烯合成酶能够催化焦磷酸法呢酯合成不同种类的倍半萜类化合物;在大肠杆菌中表达了Tri 4和Tri 11基因，并对其酶学性质进行了表征。系统发育树显示，M. rordum和其他物种。本研究为进一步研究M.防止蚜虫危害，提高经济作物产量。
Myrothecium roridum is a plant pathogenic fungus that infects different crops and decreases the yield of economical crops, including soybean, cotton, corn, pepper, and tomato. Until now, the pathogenic mechanism of M. roridum has remained unclear. Different types of trichothecene mycotoxins were isolated from M. roridum, and trichothecene was considered as a plant pathogenic factor of M. roridum. In this study, the transcriptome of M. roridum in different incubation durations was sequenced using an Illumina Hiseq 2000. A total of 35,485 transcripts and 25,996 unigenes for M. roridum were obtained from 8.0 Gb clean reads. The protein–protein network of the M. roridum transcriptome indicated that the mitogen-activated protein kinases signal pathway also played an important role in the pathogenicity of M. roridum. The genes related to trichothecene biosynthesis were annotated. The expression levels of these genes were also predicted and validated through quantitative real-time polymerase chain reaction. Tri5 gene encoding trichodiene synthase was cloned and expressed, and the purified trichodiene synthase was able to catalyze farnesyl pyrophosphate into different kinds of sesquiterpenoids.Tri4 and Tri11 genes were expressed in Escherichia coli, and their corresponding enzymatic properties were characterized. The phylogenetic tree of trichodiene synthase showed a great discrepancy between the trichodiene synthase from M. roridum and other species. Our study on the genes related to trichothecene biosynthesis establishes a foundation for the M. roridum hazard prevention, thus improving the yields of economical crops.