A two-injection protocol for nicotine sensitization
A two-injection protocol for nicotine sensitization
复制标题
DOI：
10.1016/j.bbr.2014.08.048
复制
发表时间：
2014-12-15
影响因子：
2.7
通讯作者：
Spanagel, Rainer
中科院分区：
文献类型：
--
作者：
Bernardi, Rick E.;Spanagel, Rainer
对滥用药物的运动激活效应的敏感化发生在反复接触一种药物后，和/或当有限地接触一种药物与特定环境配对时。条件性或上下文相关的敏化已经在例如可卡因和安非他明处理的动物中使用有限的暴露方案得到了很好的表征。然而，关于尼古丁等其他滥用药物的有限暴露方案的数据很少。目前的实验调查了两次注射尼古丁的方案是否会导致运动敏化。两次注射尼古丁(0.175 mg/kg，S.C.)相隔7天后，小鼠对第二次注射表现出明显的运动敏感化。此外，在第一次接触尼古丁之前，给予烟碱型乙酰胆碱受体拮抗剂甲氨基甲胺(2 mg/kg)，可阻止这种敏化的发展。在一项后续研究中，我们发现这种两次注射尼古丁的敏化作用与环境无关，因为不同组的小鼠给予初始尼古丁暴露(0.175 mg/kg，S.C.)。无论是在测试运动活动的特定环境中，还是在7天后的后续测试中，笼子中的动物都显示出同等水平的运动活动。这些数据表明了一种利用有限的尼古丁暴露来实现尼古丁敏化的新方法。(C)2014爱思唯尔B.V.保留所有权利。
Sensitization to the locomotor activating effect of drugs of abuse occurs following repeated exposure to a drug, and/or when limited exposure to a drug is paired with a specific environment. Conditioned, or context-dependent, sensitization has been well-characterized using limited exposure protocols in, for example, cocaine- and amphetamine-treated animals. However, little data exists regarding limited exposure protocols for other drugs of abuse, such as nicotine. The current experiment investigated whether a two-injection protocol of nicotine administration would result in locomotor sensitization. Mice administered two injections of nicotine (0.175 mg/kg, s.c.) 7 d apart demonstrated significant locomotor sensitization in response to the second exposure. Furthermore, the development of this sensitization was blocked by the administration of the nicotinic acetylcholine receptor antagonist mecamylamine (2 mg/kg) prior to the first nicotine exposure. In a follow-up study, we found that this two-injection nicotine sensitization was independent of context, as separate groups of mice given an initial nicotine exposure (0.175 mg/kg, s.c.) in either the specific environment in which locomotor activity was tested or in their home cages demonstrated equivalent levels of locomotor activity during subsequent testing 7 d later. These data suggest a novel approach to nicotine sensitization using limited nicotine exposure. (C) 2014 Elsevier B.V. All rights reserved.