Filamentous growth of Candida albicans in response to physical environmental cues and its regulation by the unique CZF1 gene.
Filamentous growth of Candida albicans in response to physical environmental cues and its regulation by the unique CZF1 gene.
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DOI：
10.1046/j.1365-2958.1999.01619.x
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发表时间：
1999-01-01
影响因子：
3.6
通讯作者：
Chen, X.
中科院分区：
文献类型：
--
作者：
Brown, D. H., Jr.;Giusani, A. D.;Chen, X.
在这项研究中，证明了物理环境的一个特征对白色念珠菌菌丝生长的刺激。具体来说，细胞在基质内的生长促进了菌丝的形成。编码一种转录因子的CZF1基因被证明在某些条件下，包括包埋条件下，参与了菌丝生长的调控。CZF1在包埋细胞中的异位表达促进了菌丝的快速形成。去除编码酿酒酵母Ste12p转录因子同源物的CZF1和CPH1，会导致包埋细胞的丝状生长出现明显缺陷。在某些条件下，包括包埋条件下，单独消除CZF1会导致菌丝生长的中度缺陷。这表明白色念珠菌可能在基质包埋后发生菌丝形态发生，从而促进真菌细胞侵入宿主组织。
In this study, stimulation of C. albicans hyphal growth by a feature of the physical environment was demonstrated. Specifically, growth of cells embedded within a matrix promoted the formation of hyphae. The CZF1 gene, encoding a putative transcription factor, was shown to be involved in the regulation of hyphal growth under certain conditions, including embedded conditions. Ectopic expression of CZF1 in embedded cells promoted the rapid formation of hyphae. Elimination of CZF1 and CPH1, encoding a homologue of the Saccharomyces cerevisiae Ste12p transcription factor, led to a pronounced defect in filamentous growth of embedded cells. Elimination of CZF1 alone led to a moderate defect in hyphal growth under some conditions, including embedded conditions. It is suggested that hyphal morphogenesis in response to matrix embedding may occur in C. albicans to promote invasion of fungal cells into host tissue.