Adjuvant treatment with tumor-targeting Salmonella typhimurium A1-R reduces recurrence and increases survival after liver metastasis resection in an orthotopic nude mouse model.
Adjuvant treatment with tumor-targeting Salmonella typhimurium A1-R reduces recurrence and increases survival after liver metastasis resection in an orthotopic nude mouse model.
复制标题
DOI：
10.18632/oncotarget.6170
复制
发表时间：
2015-12-08
期刊：
影响因子：
--
通讯作者：
Hoffman RM
中科院分区：
文献类型：
--
作者：
Murakami T;Hiroshima Y;Zhao M;Zhang Y;Chishima T;Tanaka K;Bouvet M;Endo I;Hoffman RM
结肠癌肝转移通常是这种疾病致命的方面。孤立的转移瘤适合手术切除，但复发很常见。因此，需要更好的辅助治疗来减少或防止复发。本研究利用表达红色荧光蛋白(RFP)的人结肠癌细胞株HT-29建立裸鼠肝转移模型。将单发肝转移的小鼠随机分为强光手术组(BLS)和联合应用肿瘤靶向鼠伤寒沙门氏菌A1-R的联合治疗组。高倍镜下荧光成像可清晰显示BLS术后残留肿瘤荧光。鼠伤寒沙门氏菌A1-R辅助治疗可显著提高肝转移瘤术后的生存率和无瘤生存率。本研究结果提示，肝转移癌切除后应用鼠伤寒沙门氏菌A1-R佐剂治疗的临床应用前景。
Colon cancer liver metastasis is often the lethal aspect of this disease. Well-isolated metastases are candidates for surgical resection, but recurrence is common. Better adjuvant treatment is therefore needed to reduce or prevent recurrence. In the present study, HT-29 human colon cancer cells expressing red fluorescent protein (RFP) were used to establish liver metastases in nude mice. Mice with a single liver metastasis were randomized into bright-light surgery (BLS) or the combination of BLS and adjuvant treatment with tumor-targeting S. typhimurium A1-R. Residual tumor fluorescence after BLS was clearly visualized at high magnification by fluorescence imaging. Adjuvant treatment with S. typhimurium A1-R was highly effective to increase survival and disease-free survival after BLS of liver metastasis. The results suggest the future clinical potential of adjuvant S. typhimurium A1-R treatment after liver metastasis resection.