3D printed porous PLA/nHA composite scaffolds with enhanced osteogenesis and osteoconductivity in vivo for bone regeneration
3D printed porous PLA/nHA composite scaffolds with enhanced osteogenesis and osteoconductivity in vivo for bone regeneration
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DOI：
10.1088/1748-605x/ab388d
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发表时间：
2019-11-01
影响因子：
4
通讯作者：
Chen, Gang
中科院分区：
文献类型：
--
作者：
Chen, Xibao;Gao, Chunxia;Chen, Gang
大型骨缺损的修复和再生仍然是一个挑战，特别是对于不规则和复杂的骨缺损。三维打印作为一种先进的制造技术，因其高精度、可定制的几何形状和个性化而受到广泛关注。本研究通过桌面熔融沉积建模技术，成功制备了具有增强成骨和骨导电性的三维多孔聚乳酸/纳米羟基磷灰石（PLA/nHA）复合支架。形态、组成和结构分析表明，nHA被成功引入PLA体系，并均匀分散在打印的PLA/nHA支架中。体外抗菌实验证实，打印的多孔PLA/nHA支架具有良好的加载和释放万古霉素和左氧氟沙星的能力。同时，通过MG-63细胞的增殖和细胞形态学分析，评价打印多孔PLA/nHA支架的细胞相容性。建立家兔模型，评价PLA/ n哈斯支架的成骨性和骨导电性。这些结果表明，3D打印PLA/nHA支架具有修复和再生大型骨缺损的巨大潜力。
Repair and regeneration of large bone defects is still a challenge, especially for defects which are the irregular and complex. Three-dimension (3D) printing, as an advanced fabrication technology, has been received considerable attentions due to its high precision, customized geometry and personalization. In this study, 3D porous polylactic acid/nano hydroxyapatite (PLA/nHA) composite scaffolds with enhanced osteogenesis and osteoconductivity were successfully fabricated by desktop fused deposition modeling technology. Morphological, composition and structural analysis revealed that nHA was successfully introduced into the PLA system and homogeneously dispersed in the printed PLA/nHA scaffolds. In vitro antibacterial experiment confirmed that the printed porous PLA/nHA scaffolds have good ability for loading and releasing vancomycin and levofloxacin. Meanwhile, MG-63 cells were used to evaluate the cytocompatibility of printed porous PLA/nHA scaffolds by proliferation and cellular morphological analysis. In addition, rabbit model was established to evaluate the osteogenesis and osteoconductivity of printed PLA/nHAscaffolds. All these results suggested that the 3D printed PLA/nHA scaffolds have great potential for repairing and regeneration of large bone defects.