COMPARISON OF QUANTITATIVE AND SEMIQUANTITATIVE CULTURE TECHNIQUES FOR BURN BIOPSY
COMPARISON OF QUANTITATIVE AND SEMIQUANTITATIVE CULTURE TECHNIQUES FOR BURN BIOPSY
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DOI：
10.1128/jcm.23.2.258-261.1986
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发表时间：
1986-02-01
影响因子：
9.4
通讯作者：
COYLE, MB
中科院分区：
文献类型：
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作者：
BUCHANAN, K;HEIMBACH, DM;COYLE, MB
细菌定植作为伤口脓毒症的预测指标的准确评价依赖于定量培养技术，该技术通过培养活检组织匀浆的五个系列稀释液提供每克组织的精确菌落计数。该方法虽然对医生有用，但既劳动密集又昂贵。在这项研究中，78焦痂活检培养的半定量技术，涉及使用0.1-和0.01-ml的接种物样品和系列稀释法。半定量培养法的精确菌落计数仅适用于含104至106 CFU/g组织的培养物。其他菌落计数报告为小于104或大于106 CFU/g。两种方法之间的菌落计数类别一致率为96%。对于伤口脓毒症的预测，半定量方法的阳性预测值为100%，阴性预测值为93.7%。该方法还导致工作单位减少约30%（由美国病理学家学会定义），用于标本处理的培养基量减少60%。因此，这种半定量培养技术为医生提供了准确的信息，同时节省了时间和材料。
Accurate evaluation of bacterial colonization as a predictive index for wound sepsis has relied on a quantitative culture technique that provides exact colony counts per gram of tissue by culture of five serial dilutions of biopsy tissue homogenate. The method, while useful to the physician, is both labor intensive and expensive. In this study 78 eschar biopsies were cultured by a semiquantitative technique that involved the use of 0.1- and 0.01-ml samples of inocula and by the serial dilution method. Exact colony counts from the semiquantitative culture method were available only from cultures containing 104 to 106 CFU/g of tissue. Other colony counts were reported as less than 104 or greater than 106 CFU/g. Agreement by category of colony counts between the two methods was 96%. For prediction of wound sepsis, the semiquantitative procedure had a positive predictive value of 100% and a negative predictive value of 93.7%. This method also resulted in an approximately 30% reduction of work units (as defined by the College of American Pathologists) and a 60% reduction of the amount of media for specimen processing. Therefore, this semiquantitative culture technique provides accurate information to the physician while saving both time and materials.