Vascular endothelial growth factor, platelet-derived growth factor, and insulin-like growth factor-1 promote rat aortic angiogenesis in vitro.
Vascular endothelial growth factor, platelet-derived growth factor, and insulin-like growth factor-1 promote rat aortic angiogenesis in vitro.
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发表时间：
1994-11
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通讯作者：
R. Nicosia;S. Nicosia;M. Smith
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作者：
R. Nicosia;S. Nicosia;M. Smith
这项研究的目的是评估分离的血管外植体对各种已被证明刺激血管生成的生长因子的血管形成反应。大鼠主动脉环在胶原凝胶中培养，加入或不加入血管内皮生长因子(VEGF)、天然血小板衍生生长因子(PDGF)、PDGF-AA、PDGF-BB、胰岛素样生长因子-1(IGF-1)、转化生长因子-α(TGF-α)、转化生长因子-β1(TGF-β1)、表皮生长因子(EGF)、白介素1α(IL-1α)或肝细胞生长因子(HGF)。血管内皮生长因子、血小板衍生生长因子、血小板衍生生长因子-AA、血小板衍生生长因子-BB和胰岛素样生长因子-1刺激大鼠主动脉血管生成反应。血管内皮生长因子和血小板衍生生长因子-BB的刺激作用最强。而转化生长因子-β1和白介素1-α则具有抑制作用。未观察到转化生长因子-α、表皮生长因子或肝细胞生长因子的显著影响。经血管内皮生长因子刺激的培养物中血管突起主要由微血管组成，而经PDGF和IGF-1刺激的培养物中含有更多的成纤维细胞样细胞。在无血清培养中，转化生长因子-α、转化生长因子-β1、白介素1-α、表皮生长因子和肝细胞生长因子不能刺激大鼠主动脉血管生成，这表明这些因素需要大鼠主动脉模型中不存在的辅助细胞的中介活动，或者血管对不同的血管生成因子的反应能力不同。
The purpose of this study was to evaluate the vasoformative response of isolated vascular explants to a variety of growth factors that have been shown to stimulate angiogenesis. Rings of rat aorta were cultured in collagen gels under serum-free conditions in the presence or absence of vascular endothelial growth factor (VEGF), natural platelet-derived growth factor (PDGF), PDGF-AA, PDGF-BB, insulin-like growth factor-1 (IGF-1), transforming growth factor-alpha (TGF-alpha), transforming growth factor-beta 1 (TGF-beta 1), epidermal growth factor (EGF), interleukin-1 alpha (IL-1 alpha), or hepatocyte growth factor (HGF). The angiogenic response of the rat aorta was stimulated by VEGF, PDGF, PDGF-AA, PDGF-BB, and IGF-1. Maximum stimulatory effects were obtained with VEGF and PDGF-BB. By contrast, TGF-beta 1 and IL-1 alpha had inhibitory activity. No significant effects were observed with TGF-alpha, EGF, or HGF. The vascular outgrowth of VEGF-stimulated cultures was primarily composed of microvessels, whereas that of PDGF- and IGF-1-stimulated cultures contained an increased number of fibroblast-like cells. The inability of TGF-alpha, TGF-beta 1, IL-1 alpha, EGF, and HGF to stimulate rat aortic angiogenesis in serum-free culture suggests that either these factors require the mediatory activity of accessory cells that are not present in the rat aorta model or that blood vessels are heterogeneous in their capacity to respond to different angiogenic factors.