Incorporation of a Reporter Peptide in FPOP Compensates for Adventitious Scavengers and Permits Time-Dependent Measurements
Incorporation of a Reporter Peptide in FPOP Compensates for Adventitious Scavengers and Permits Time-Dependent Measurements
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DOI：
10.1007/s13361-016-1552-4
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发表时间：
2017-02-01
影响因子：
3.2
通讯作者：
Gross, Michael L.
中科院分区：
文献类型：
--
作者：
Niu, Ben;Mackness, Brian C.;Gross, Michael L.
在进行蛋白质快速光化学氧化（FPOP）的溶液中掺入报告肽，可以校正外源清除剂，并使时间依赖性结果的标准化和比较成为可能。报道者也将在差异实验中有用，以控制自由基反应物种的包含。这种结合提供了一个简单而快速的检查自由基剂量，并允许比较FPOP结果从日常和实验室到实验室。在FPOP工作流程中使用报告肽不需要额外的测量或光谱仪，同时构建更定量的FPOP平台。它只需要在LC/MS/MS运行中测量肽修饰的程度，这是通过使用数据依赖性扫描或包含列表来进行的。
Incorporation of a reporter peptide in solutions submitted to fast photochemical oxidation of proteins (FPOP) allows for the correction of adventitious scavengers and enables the normalization and comparison of time-dependent results. Reporters will also be useful in differential experiments to control for the inclusion of a radical-reactive species. This incorporation provides a simple and quick check of radical dosage and allows comparison of FPOP results from day-to-day and lab-to-lab. Use of a reporter peptide in the FPOP workflow requires no additional measurements or spectrometers while building a more quantitative FPOP platform. It requires only measurement of the extent of reporter-peptide modification in a LC/MS/MS run, which is performed by using either data-dependent scanning or an inclusion list.