Transcriptome profiles of red swamp crayfish Procambarus clarkii hematopoietic tissue in response to WSSV challenge
Transcriptome profiles of red swamp crayfish Procambarus clarkii hematopoietic tissue in response to WSSV challenge
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克氏原螯虾造血组织响应 WSSV 挑战的转录组谱
DOI：
10.1016/j.fsi.2022.01.041
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发表时间：
2022-02-11
影响因子：
4.7
通讯作者：
Liu, Qiuning
中科院分区：
文献类型：
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作者：
Yang, Hui;Ji, Tongwei;Liu, Qiuning
克氏原虾在感染WSSV后可获得较高的累积死亡率。为了更好地了解WSSV在造血组织中的免疫应答，本研究对克氏疟原虫的免疫应答进行了研究，并分析了一些造血细胞因子的表达。组装后，对照组和治疗组平均获得47,712,411个清洁读数。共发现35,945个单基因，其N50长度为1554个碱基。利用不同的数据库进行功能分类、浓缩和通径分析，共鉴定出约257个差异表达基因，其中139个上调，118个下调。GO功能分析主要参与免疫反应的激活、补体的激活、补体结合、体液免疫反应的负调节和分泌颗粒膜。KEGG分析表明，这些DEG参与了细胞外基质-受体相互作用、HIF-1信号通路、糖酵解/糖异生、甲状腺激素信号通路和胰高血糖素信号通路。对筛选出的9个基因进行实时定量聚合酶链式反应(RT-qPCR)分析，证实了RNA-Seq结果的可靠性。本研究首次提供了克氏疟原虫造血组织对WSSV感染反应的转录图谱，并揭示了Aspakines可能在抗病毒免疫反应中发挥重要作用。本研究成果将进一步丰富小龙虾免疫系统的理论基础，为疾病防控提供新的思路。
The crayfish Procambarus clarkii could achieve a high cumulative mortality after WSSV infections. To better understand the immune response to WSSV in hematopoietic tissue, the present study investigated the immu-nological response of P. clarkii and analyzed the expression of some hematopoietic cytokines. After assembly, there was an average of 47,712,411 clean reads were obtained in control and treatment groups. A total of 35,945 unigenes were discovered with N50 length of 1554 bp. Under functional classification, enrichment, and pathway analysis using different database, there were about 257 differentially expressed genes (DEGs) identified, of which 139 were up-regulated and 118 were down-regulated. The GO function analysis of these DEGs were mostly participated in activation of immune response, complement activation, complement binding, negative regulation of humoral immune response and secretory granule membrane. Under KEGG analysis, these DEGs were involved in ECM-receptor interaction, HIF-1 signaling pathway, Glycolysis/Gluconeogenesis, Thyroid hormone signaling pathway and Glucagon signaling pathway. The real-time quantitative PCR (RT-qPCR) analysis of 9 selected genes confirmed the reliability of RNA-Seq results. The present research provide for the first time the transcriptomic profile of P. clarkii hematopoietic tissue in response to WSSV infection and reveals the astakines may play important roles in antiviral immune response. The results of the present study will further enrich the theoretical basis of the crayfish immune system and provide new ideas for disease prevention and control.