Impact of Hepatic Arterial Reconstruction on Orthotopic Liver Transplantation in the Rat
Impact of Hepatic Arterial Reconstruction on Orthotopic Liver Transplantation in the Rat
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DOI：
10.3109/08941939.2011.636476
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发表时间：
2012-08-01
影响因子：
1.9
通讯作者：
Nguyen, Justin H.
中科院分区：
文献类型：
--
作者：
Hori, Tomohide;Gardner, Lindsay B.;Nguyen, Justin H.
许多研究已经对大鼠原位肝移植（OLT）模型进行了研究，但肝动脉（HA）重建对术后因素影响的详细信息仍有待研究。 HA重建也需要先进的技能。研究了在全肝同基因移植大鼠中采用手工缝合技术重建 HA 的效果。长期生存、组织病理学评估、免疫组织学评估和血液生化研究一直持续到术后第28天。从术后早期开始，有或没有HA重建的OLT在移植物实质损伤、细胞凋亡诱导和转氨酶水平方面发现显着差异，但生存曲线和凝血曲线没有显示差异。在没有HA重建的OLT中，胆道增殖在POD 5-14时减少，总胆红素水平在POD 10和14时增加。该研究表明，HA重建对于大鼠可靠的OLT是必需的。
Orthotopic liver transplantation (OLT) models in rats have been investigated in many studies, but detailed information on the impact of hepatic artery (HA) reconstruction on postoperative factors remains to be investigated. HA reconstruction also requires advanced skills. The effect of the reconstruction of the HA by a hand-suture technique in rats with a whole-liver syngeneic graft was investigated. Long-term survival, histopathological assessment, immunohistological evaluation, and blood biochemistry were investigated until postoperative day (POD) 28. From the early postoperative period, significant differences between OLTs with or without HA reconstruction were found in graft parenchymal damage, induction of apoptosis, and transaminase levels, though survival curves and the coagulation profile showed no differences. In OLT without HA reconstruction, biliary proliferation was decreased at POD 5-14, and total bilirubin level was increased at PODs 10 and 14. The study indicates that HA reconstruction is required for reliable OLT in rats.