Collection of SARS-CoV-2 Virus from the Air of a Clinic within a University Student Health Care Center and Analyses of the Viral Genomic Sequence
Collection of SARS-CoV-2 Virus from the Air of a Clinic within a University Student Health Care Center and Analyses of the Viral Genomic Sequence
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DOI：
10.4209/aaqr.2020.05.0202
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发表时间：
2020-06-01
影响因子：
4
通讯作者：
Wu, Chang-Yu
中科院分区：
文献类型：
--
作者：
Lednicky, John A.;Shankar, Sripriya N.;Wu, Chang-Yu
通过识别SARS-CoV-2基因组序列中随时间发生的突变，可以跟踪全球COVID-19的进展。目前，此类工作依赖于对患者标本中的SARS-CoV-2基因组（直接测序）或从细胞培养的患者标本中分离的病毒进行基因组测序。在大学生保健中心的诊所进行的SARS-CoV-2空气抽样试点研究检测到病毒vRNA，估计浓度为0.87病毒基因组L-1空气。为了确定检测到的病毒是否有活力（“活”），尝试在细胞培养物中分离病毒。用空气样本收集培养基接种Vero E6细胞后2 d内观察到病毒诱导的细胞病变效应（CPE）；然而，细胞培养中SARS-CoV-2 vRNA的rtRT-PCR检测为阴性。相反，另外三种快速生长的人类呼吸道病毒被分离出来并随后被鉴定出来。说明了在测试样本中存在多种病毒时分离SARS-CoV-2的挑战。然而，完整的SARS-CoV-2基因组序列是通过Sanger测序确定的，与先前在美国佐治亚州描述的SARS-CoV-2基因组最接近。本研究结果说明了利用环境气溶胶样本代替人体样本跟踪COVID-19大流行进展的可行性。在距离最近的病人交通超过2米的地方采集阳性样本表明病毒是以气溶胶形式存在的。
The progression of COVID-19 worldwide can be tracked by identifying mutations within the genomic sequence of SARS-CoV-2 that occur as a function of time. Such efforts currently rely on sequencing the genome of SARS-CoV-2 in patient specimens (direct sequencing) or of virus isolated from patient specimens in cell cultures. A pilot SARS-CoV-2 air sampling study conducted at a clinic within a university student health care center detected the virus vRNA, with an estimated concentration of 0.87 virus genomes L-1 air. To determine whether the virus detected was viable ('live'), attempts were made to isolate the virus in cell cultures. Virus-induced cytopathic effects (CPE) were observed within two days post-inoculation of Vero E6 cells with collection media from air samples; however, rtRT-PCR tests for SARS-CoV-2 vRNA from cell culture were negative. Instead, three other fast-growing human respiratory viruses were isolated and subsequently identified. illustrating the challenge in isolating SARS-CoV-2 when multiple viruses are present in a test sample. The complete SAR-CoV-2 genomic sequence was nevertheless determined by Sanger sequencing and most closely resembles SARS-CoV-2 genomes previously described in Georgia, USA. Results of this study illustrate the feasibility of tracking progression of the COVID-19 pandemic using environmental aerosol samples instead of human specimens. Collection of a positive sample from a distance more than 2 m away from the nearest patient traffic implies the virus was in an aerosol.