Modulating macrophage polarization with divalent cations in nanostructured titanium implant surfaces
Modulating macrophage polarization with divalent cations in nanostructured titanium implant surfaces
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DOI：
10.1088/0957-4484/27/8/085101
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发表时间：
2016-02-26
期刊：
影响因子：
3.5
通讯作者：
Park, Jin-Woo
中科院分区：
文献类型：
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作者：
Lee, Chung-Ho;Kim, Youn-Jeong;Park, Jin-Woo
利用生物活性离子进行纳米级形貌改性和表面化学改变是当前设计具有增强骨愈合能力的钛（Ti）骨植入物表面的重要过程。巨噬细胞在早期组织愈合阶段发挥核心作用，已知它们对植入物表面的反应活性会影响随后的愈合结果。因此，修饰表面对巨噬细胞表型极化（即朝向再生M2而不是炎症M1表型）的正调节对于钛骨植入物的成骨功能至关重要。然而，在钛骨植入物的表面设计中，针对纳米形貌和生物活性离子化学这两个重要的表面特性，在调节以巨噬细胞为中心的早期愈合能力方面取得的进展相对较少。我们研究了表面生物活性离子改性与Ti的表面纳米形貌相结合时，是否对诱导再生M2巨噬细胞极化产生一定的有益影响。我们的研究结果表明，纳米级的形貌修饰和表面生物活性离子化学可以积极调节钛植入物表面的巨噬细胞表型。据我们所知，这是第一次证明使用二价阳离子（Ca和Sr）的化学表面改性显著诱导J774.A1细胞在纳米结构Ti表面的再生M2巨噬细胞表型。在这项研究中，二价阳离子化学调节粘附巨噬细胞的细胞形状，并显着上调M2巨噬细胞表型表达时，与纳米结构的钛表面。这些结果提供了对未来钛骨植入物表面工程的深入了解，这些表面工程在巨噬细胞控制的早期伤口愈合过程和随后的以间充质干细胞为中心的成骨功能之间协调。
Nanoscale topographical modification and surface chemistry alteration using bioactive ions are centrally important processes in the current design of the surface of titanium (Ti) bone implants with enhanced bone healing capacity. Macrophages play a central role in the early tissue healing stage and their activity in response to the implant surface is known to affect the subsequent healing outcome. Thus, the positive modulation of macrophage phenotype polarization (i.e. towards the regenerative M2 rather than the inflammatory M1 phenotype) with a modified surface is essential for the osteogenesis funtion of Ti bone implants. However, relatively few advances have been made in terms of modulating the macrophage-centered early healing capacity in the surface design of Ti bone implants for the two important surface properties of nanotopography and and bioactive ion chemistry. We investigated whether surface bioactive ion modification exerts a definite beneficial effect on inducing regenerative M2 macrophage polarization when combined with the surface nanotopography of Ti. Our results indicate that nanoscale topographical modification and surface bioactive ion chemistry can positively modulate the macrophage phenotype in a Ti implant surface. To the best of our knowledge, this is the first demonstration that chemical surface modification using divalent cations (Ca and Sr) dramatically induces the regenerative M2 macrophage phenotype of J774.A1 cells in nanostructured Ti surfaces. In this study, divalent cation chemistry regulated the cell shape of adherent macrophages and markedly up-regulated M2 macrophage phenotype expression when combined with the nanostructured Ti surface. These results provide insight into the surface engineering of future Ti bone implants that are harmonized between the macrophage-governed early wound healing process and subsequent mesenchymal stem cell-centered osteogenesis function.