Lycopene intake induces colonic regulatory T cells in mice and suppresses food allergy symptoms.
Lycopene intake induces colonic regulatory T cells in mice and suppresses food allergy symptoms.
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摄入番茄红素可诱导小鼠结肠调节 T 细胞并抑制食物过敏症状。
DOI：
10.1111/pai.13691
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Naito Y.
中科院分区：
文献类型：
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作者：
Ushiroda C;Takagi T;Fuke N;Mizushima K;Hirai Y;Higashimura Y;Harusato A;Kamada K;Uchiyama K;Ishikawa T;Aizawa K;Suganuma H;Itoh Y;Naito Y.
研究背景食物过敏（food allergies，FA）是儿童常见病，对其预防和治疗要求较高的安全性。结肠调节性T细胞（TCRs）已被认为可以减弱FA。我们研究了蔬菜和水果中含有的色素类胡萝卜素的Treg诱导能力和抗FA作用。方法C57 BL/6 N小鼠饲喂含有0.01%（w/w）番茄红素、β-胡萝卜素、虾青素或叶黄素的饲料4周，并评估结肠Tlag的数量。随后，为了评价番茄红素的Treg诱导能力，将来自BALB/c小鼠的脾幼稚CD 4 +T细胞与抗CD 3/CD 28抗体、TGF-β和番茄红素一起培养，并检查Treg的频率。在OVA诱导的FA模型BALB/c小鼠中研究了含有0.1%（w/w）番茄红素的饮食对FA的影响。番茄红素还增加脾脏幼稚CD 4 +T细胞中的Treg分化。在FA小鼠中，番茄红素喂养显着增加结肠T细胞的数量和减弱过敏症状。番茄红素还可显著降低结肠黏膜中IL-4、IL-9和IL-13 mRNA的表达水平。IL-9是已知的诱导肥大细胞的增殖，我们发现，番茄红素喂养显着减少肥大细胞的数量在结肠粘膜的FA mouse.ConclusionOur结果表明，番茄红素，类胡萝卜素存在于许多常见的食品在市场上，可能有可能诱导结肠Tumor和抑制FA症状。
BackgroundFood allergy (FA) is a common disease in children; thus, a high level of safety is required for its prevention and treatment. Colonic regulatory T cells (Tregs) have been suggested to attenuate FA. We investigated the Treg‐inducing ability and anti‐FA effects of carotenoids, a pigment contained in vegetables and fruits.MethodsC57BL/6N mice were fed a diet containing 0.01% (w/w) of lycopene, β‐carotene, astaxanthin or lutein for 4 weeks, and the population of colonic Tregs was assessed. Subsequently, to evaluate the Treg‐inducing ability of lycopene, splenic naïve CD4+T cells from BALB/c mice were cultured with anti‐CD3/CD28 antibody, TGF‐β and lycopene, and the frequencies of Tregs were examined. The effect of 0.1% (w/w) lycopene containing diet on FA was investigated in OVA‐induced FA model BALB/c mice.ResultsIn screening, only lycopene significantly increased the frequency and number of colonic Tregs. Lycopene also increased Treg differentiation in splenic naïve CD4+T cells. In FA mice, lycopene feeding significantly increased the number of colonic Tregs and attenuated allergic symptoms. The expression levels of IL‐4, IL‐9 and IL‐13 mRNA in colonic mucosa were also significantly reduced by lycopene. IL‐9 is known to induce proliferation of mast cells, and we found that lycopene feeding significantly reduced the number of mast cells in the colonic mucosa of FA mice.ConclusionOur results suggest that lycopene, a carotenoid present in many common foods on the market, may have the potential to induce colonic Tregs and suppress FA symptoms.