Identification and classification of silks using infrared spectroscopy.
Identification and classification of silks using infrared spectroscopy.
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DOI：
10.1242/jeb.128306
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发表时间：
2015-10
期刊：
影响因子：
--
通讯作者：
Holland C
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文献类型：
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作者：
Boulet-Audet M;Vollrath F;Holland C
鳞翅目蛛丝的数量多达数千条，显示出结构、性能和工业潜力的巨大多样性。为了描绘这种显著的生化多样性，我们提出了一种基于土丝原料和茧红外光谱的鉴定和筛选方法。通过对35种蚕丝的1214个红外光谱进行多元分析，我们将蚕丝划分为不同的等级，这一分类与目前的系统发育数据和分类非常一致。该方法还提供了丝胶、草酸钙、酚类化合物、聚丙氨酸和聚丙氨酸-甘氨酸β-片的相对含量信息。驯化的桑蚕家蚕在光谱特性方面与其他家蚕类群相比是一个异类。有趣的是，据报道，到目前为止，在任何野生蚕蛾中，紫荆粉含有最多的β-片。我们的结论是，我们的方法为确定蚕茧的化学成分提供了一条新的途径，从而为蚕丝的生物学和材料分类提供了一种新的途径。摘要：对35个不同物种的未纺蚕丝和茧进行了FTIR分析，为这类材料的生化多样性和进化提供了信息。
Lepidopteran silks number in the thousands and display a vast diversity of structures, properties and industrial potential. To map this remarkable biochemical diversity, we present an identification and screening method based on the infrared spectra of native silk feedstock and cocoons. Multivariate analysis of over 1214 infrared spectra obtained from 35 species allowed us to group silks into distinct hierarchies and a classification that agrees well with current phylogenetic data and taxonomies. This approach also provides information on the relative content of sericin, calcium oxalate, phenolic compounds, poly-alanine and poly(alanine-glycine) β-sheets. It emerged that the domesticated mulberry silkmoth Bombyx mori represents an outlier compared with other silkmoth taxa in terms of spectral properties. Interestingly, Epiphora bauhiniae was found to contain the highest amount of β-sheets reported to date for any wild silkmoth. We conclude that our approach provides a new route to determine cocoon chemical composition and in turn a novel, biological as well as material, classification of silks. Summary: FTIR analysis performed on unspun silks and cocoons from 35 different species provides information regarding the biochemical diversity and evolution of this group of materials.