Molecular identification and phylogeny of cucumber mosaic virus and zucchini yellow mosaic virus co-infecting Luffa cylindricaL. in Shanxi
Molecular identification and phylogeny of cucumber mosaic virus and zucchini yellow mosaic virus co-infecting Luffa cylindricaL. in Shanxi
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黄瓜花叶病毒和西葫芦黄花叶病毒共感染丝瓜的分子鉴定及系统发育。
DOI：
10.1007/s42161-019-00480-7
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发表时间：
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影响因子：
2.2
通讯作者：
YanBing Niu
中科院分区：
文献类型：
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作者：
Defu Wang;JiangRan Wang;LiYan Cui;ShuTing Wang;YanBing Niu
鉴定引起丝瓜枯萎病的病毒。在花叶病毒病中，提取病毒基因组双链RNA（dsRNA），然后进行序列非依赖性扩增（SIA）和测序。结果表明：丝瓜（Luffa cylindrica L.）黄瓜花叶病毒（CMV）和小西葫芦黄化花叶病毒（ZYMV）共感染的。为了进一步研究黄瓜花叶病毒（CMV-SXSG）和小西葫芦黄化花叶病毒（ZYMV-SXSG）山西分离物的特性，克隆了CMV-SXSG MP基因序列和ZYMV-SXSG全基因组序列。ZYMV-SXSG基因组RNA全长9474个核苷酸，含有一个开放阅读框，编码3080个氨基酸的多聚蛋白。序列比对结果表明，CMV-SXSG与其它CMV分离物MP核苷酸同源性为78.15%~ 99.4%。SXSG株系与ZYMV代表株系CDS区核苷酸序列的相似性为81.71%~ 98.40%。系统发育分析表明，CMV-SXSG与CMV-QZ同源，ZYMV-SXSG与KR-PA分离物序列同源性最高，形成独立的分支，表明它们的进化关系最为密切。结果表明，ZYMV-SXSG为重组体，其主要亲本为ZZ（中国分离物），次要亲本为KR-PA（韩国分离物）。这些结果将为更准确地分析CMV或ZYMV的遗传多样性提供有价值的线索，也为设计更好的病毒控制策略提供有效的理论依据。
To identify the viruses that inducedLuffa cylindricaL. mosaic disease, the viral genomic double-strand RNA (dsRNA) was extracted, followed by sequence-independent amplification (SIA) and sequencing. Results showed thatLuffa cylindricaL. was co-infected by cucumber mosaic virus (CMV) and zucchini yellow mosaic virus (ZYMV). To further characterize the Shanxi isolates of cucumber mosaic virus (CMV-SXSG) and zucchini yellow mosaic virus (ZYMV-SXSG), the MP gene sequence of CMV-SXSG and the complete genomic sequence of ZYMV-SXSG were cloned and analyzed. The genomic RNA of ZYMV-SXSG is 9474 nucleotides in length and contains one putative ORF which encodes a polyprotein of 3080 amino acids. Sequence alignments showed that CMV-SXSG shared MP nucleotide sequence identities with other CMV isolates of 78.15% to 99.4%. The nucleotide sequence similarity for the CDS region between ZYMV-SXSG and representative ZYMV strains ranged from 81.71% to 98.40%. Phylogenetic analysis suggested that CMV-SXSG and CMV-QZ shared the same origin, and that ZYMV-SXSG showed the highest sequence identity to isolate KR-PA and formed an independent branch, indicating that they have the most closely evolutionary relationship. Recombination analysis revealed that ZYMV-SXSG was a putative recombinant, and that the potential major and minor parents were the ZZ (China isolate) and KR-PA (Korean isolate), respectively. These results will provide valuable hints for more accurate analysis of CMV or ZYMV genetic diversity, and also provide an effective theoretical basis for designing better virus control strategies.