Isolation, chemical investigation and antiviral activity of polysaccharides from Gracilaria corticata (Gracilariaceae, Rhodophyta)
Isolation, chemical investigation and antiviral activity of polysaccharides from Gracilaria corticata (Gracilariaceae, Rhodophyta)
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DOI：
10.1016/s0141-8130(02)00070-3
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发表时间：
2002-12-20
影响因子：
8.2
通讯作者：
Ray, B
中科院分区：
文献类型：
--
作者：
Mazumder, S;Ghosal, PK;Ray, B
从木本植物葛缕子中依次提取多糖。化学分析结合红外光谱分析表明，冷水萃取物主要由高分子量硫酸酸化半乳组成。大多数硫酸盐基是碱不稳定的，位于1,3连接的d -半乳糖单元的C-4和1,4连接的l -半乳糖残基的C-6上。蒸压提取物含有高丙酮酸含量和不同程度甲基化的琼脂型多糖，但被佛罗里达淀粉污染。h -1核磁共振研究表明，甲氧基出现在琼脂聚合物的1,3-链d -半乳糖单元的C-6和1,4-链l -半乳糖残基的C-2上。生物测定表明，高分子量硫酸半乳聚糖对1型和2型单纯疱疹病毒表现出选择性抗病毒活性，可能是由于抑制了病毒对宿主细胞的初始附着。(C) 2002 Elsevier Science B.V.版权所有
Polysaccharides were sequentially extracted from the agarophyte Gracilaria corticata. Chemical analysis combined with infrared spectroscopy showed that the cold water extracted material consists mainly of a high molecular weight sulfated galactan. Most of the sulfate groups are alkali labile and are located at C-4 of the 1,3-linked D-galactose units and C-6 of the 1,4-linked L-galactose residues. The autoclaved extracts contain agar type polysaccharide having a high pyruvate content and a variable degree of methylation, but were contaminated with floridean starch. H-1-NMR studies indicate that methoxyl groups, when present, occur at C-6 of the 1,3-linked D-galactose units and C-2 of the 1,4-linked L-galactose residues of agar polymer. Bioassays showed that a high molecular weight galactan sulfate, exhibited selective antiviral activity against herpes simplex virus types I and 2, likely due to an inhibition of the initial virus attachment to the host cell. (C) 2002 Elsevier Science B.V. All rights reserved.