Synaptophysin regulates the kinetics of synaptic vesicle endocytosis in central neurons.
Synaptophysin regulates the kinetics of synaptic vesicle endocytosis in central neurons.
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DOI：
10.1016/j.neuron.2011.04.001
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发表时间：
2011-06-09
期刊：
影响因子：
16.2
通讯作者：
Chapman ER
中科院分区：
文献类型：
--
作者：
Kwon SE;Chapman ER
尽管是最丰富的突触囊泡膜蛋白，突触素的功能仍然是个谜。例如，据报道，突触传递在突触素敲除小鼠中是完全正常的;然而，尚未进行直接实验来监测突触囊泡周期。在这里，使用光学成像和电生理实验，我们证明，突触体素是所需的动力学有效的内吞作用的突触囊泡在培养的海马神经元。截断分析表明，不同的结构元件的突触体蛋白差异调节囊泡检索期间和刺激后。因此，突触体蛋白调节至少两个阶段的内吞作用，以确保囊泡的可用性期间和之后持续的神经元活动。
Despite being the most abundant synaptic vesicle membrane protein, the function of synaptophysin remains enigmatic. For example, synaptic transmission was reported to be completely normal in synaptophysin knockout mice; however, direct experiments to monitor the synaptic vesicle cycle have not been carried out. Here, using optical imaging and electrophysiological experiments, we demonstrate that synaptophysin is required for kinetically efficient endocytosis of synaptic vesicles in cultured hippocampal neurons. Truncation analysis revealed that distinct structural elements of synaptophysin differentially regulate vesicle retrieval during and after stimulation. Thus, synaptophysin regulates at least two phases of endocytosis to ensure vesicle availability during and after sustained neuronal activity.