Cloning and Initial Analysis of Porcine MPDU1 Gene
Cloning and Initial Analysis of Porcine MPDU1 Gene
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猪MPDU1基因的克隆及初步分析
DOI：
10.5713/ajas.2005.1237
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Kui Li
中科院分区：
文献类型：
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作者：
J. Yang;M. Yu;B. Liu;B. Fan;M. Zhu;T. Xiong;Kui Li
甘露糖-β-多萜醇利用缺陷1（MPDU 1）基因是利用甘露糖供体MPD合成脂质连接低聚糖（LLO）和糖基磷脂酰肌醇（GPI）所必需的，这对于蛋白质折叠和膜锚定等功能很重要。通过计算机克隆和cDNA末端快速扩增（RACE）技术，获得了猪MPDU 1的全长cDNA。推导的氨基酸序列与相应的人类序列具有91%的同源性，具有5个预测的跨膜区。用RT-PCR检测其在5种组织中的表达情况，结果表明其在所检测的组织中普遍表达。在外显子5内检测到导致氨基酸变化（137 Tyr-137 His）的单核苷酸取代。6个地方猪种的等位基因频率与欧洲猪种存在显著差异。利用猪/啮齿动物体细胞杂交板（SCHP），将猪MPDU 1基因定位于SSC 12，与人17号染色体和猪12号染色体之间存在保守同线群的比较定位结果一致。
Mannose-P-dolichol utilization defect 1 (MPDU1) gene is required for utilization of the mannose donor MPD in synthesis of both lipid-linked oligosaccharides (LLOs) and glycosylphosphatidylinositols (GPI) which are important for functions such as protein folding and membrane anchoring. The full length cDNA of the porcine MPDU1 was determined by in silico cloning and rapid amplification of cDNA ends (RACE). The deduced amino acid showed 91% identity to the corresponding human sequence with five predicted transmembrane regions. RT-PCR was performed to detect its expression pattern in five tissues and results showed that it is expressed ubiquitously among the tissues checked. A single nucleotide substitution resulting in the amino acid change (137 Tyr-137 His) was detected within exon 5. Allele frequencies in six pig breeds showed distinctive differences between those Chinese indigenous pigs breeds and European pigs. Using the pig/rodent somatic cell hybrid panel (SCHP), we mapped the porcine MPDU1 gene to SSC12, which is consistent with the comparative mapping result as conservative syntenic groups presented between human chromosome 17 and pig chromosome 12.