Bioethanol production from acidic and enzymatic hydrolysates of mixed microalgae culture
Bioethanol production from acidic and enzymatic hydrolysates of mixed microalgae culture
复制标题
DOI：
10.1016/j.fuel.2017.03.090
复制
发表时间：
2017-07-15
期刊：
影响因子：
7.4
通讯作者：
Zamani, Mehdi
中科院分区：
文献类型：
--
作者：
Shokrkar, Hanieh;Ebrahimi, Sirous;Zamani, Mehdi
与传统的纯培养相比，混合微藻培养被认为是一个有吸引力的研究领域，可以降低种植污染风险，提高大规模生物燃料生产的经济可行性。然而，混合微藻培养的预处理和生物乙醇生产尚未见报道。因此，本研究旨在评价不同预处理策略（酸性、碱性和酶解）对混合微藻提取糖的影响。此外，还研究了在酸性前处理中添加硫酸镁和氯化钙对还原糖产率的影响。结果表明，稀硫酸和MgSO4的混合物比稀酸的糖得率更高。在所有预处理中，热稳定性酶处理的回收率最高，为0.951 g提取葡萄糖/g总糖。此外，在相同操作条件下，对湿法微藻与干法微藻进行酶前处理进行了比较，得到了50相似的干生物质浓度的糖产量，这有利于减少对微藻生物质的干燥需求。利用酿酒酵母将酸处理和酶处理的样品发酵成乙醇的产率分别为0.38和0.46 g/g葡萄糖，分别相当于理论值的76%和92%。结果表明，混合微藻经酶解后的生物乙醇产量高于酸解后的生物乙醇产量。(C) 2017 Elsevier Ltd.版权所有。
Mixed microalgae cultures are considered as an attractive research area compared to traditional pure culture to dominate cultivation contamination risk and enhance economic feasibility of large-scale biofuel production. However, pre-treatment and bioethanol production from mixed microalgae culture has not been reported yet. Therefore, this study was aimed to evaluate the effect of different pre-treatment strategies including acidic, alkaline, and enzymatic hydrolysis on the sugar extraction from mixed microalgae. Besides, the effects of MgSO4 and CaCl2 as lewis acids in acidic pre-treatment on reducing sugar yield were studied.Results showed that the mixture of dilute sulfuric acid and MgSO4 exhibited a higher sugar yield than dilute acid. Among all pre-treatments used, the enzymatic treatment with thermostable enzymes showed the highest recovery of 0.951 g extracted glucose/g total sugar. Moreover, the enzymatic pre-treatment of wet microalgae was compared with dried ones at identical operational conditions and dried biomass concentration of 50 similar sugar yields were achieved which would be advantageous to reduce the need for drying of the microalgae biomass. Fermentation of the acidic and enzymatic treated samples to ethanol using Saccharomyces cerevisiae showed yield of 0.38 and 0.46 g/g glucose, corresponding to 76% and 92% of the theoretical values, respectively. The obtained results revealed that bioethanol yield after enzymatic hydrolysis of mixed microalgae culture are higher than those of acid hydrolysis. (C) 2017 Elsevier Ltd. All rights reserved.