TOPOGRAPHICAL AND CYTOLOGICAL EVOLUTION OF THE GLIAL PHASE DURING PRENATAL DEVELOPMENT OF THE HUMAN-BRAIN - HISTOCHEMICAL AND ELECTRON-MICROSCOPIC STUDY
TOPOGRAPHICAL AND CYTOLOGICAL EVOLUTION OF THE GLIAL PHASE DURING PRENATAL DEVELOPMENT OF THE HUMAN-BRAIN - HISTOCHEMICAL AND ELECTRON-MICROSCOPIC STUDY
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DOI：
10.1097/00005072-198803000-00009
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发表时间：
1988-03-01
影响因子：
3.2
通讯作者：
EVRARD, P
中科院分区：
文献类型：
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作者：
KADHIM, HJ;GADISSEUX, JF;EVRARD, P
利用还原型锇和过碘酸-硫代碳酰肼-银蛋白选择性染色胶质细胞群的糖原含量，对发育中的胎儿脑中产前胶质细胞发生和神经元-胶质细胞关系进行超微结构分析。在人类胎儿中证实了亲胶质神经元迁移，放射状胶质纤维（RGF）在预期的新皮质和潜在的中间区（IZ）中充当神经元迁移的强制性走廊。用这种方法，整个神经胶质相分化的神经元元素，这允许描述的进化分布模式的RGF：在皮质板，神经胶质束完全解离的18周妊娠，而在IZ，他们仍然分组束，直到他们转化为星形胶质细胞。在妊娠21至30周之间的成熟胶质细胞质中观察到的最显著和恒定的发育特征是在RGF中观察到的溶酶体器和自噬空泡的丰度和活性的根本性增强，这是放射状胶质细胞转化为星形胶质细胞的细胞学基础。这些数据的理解的个体发育的新皮层垂直模块在人类大脑和垂直皮层单位的比较哺乳动物解剖学方面的系统发育分析的影响。
An ultrastructural analysis of prenatal gliogenesis and neuronal-glial relationships in the developing fetal brain was carried out using reduced osmium and periodic acid-thiocarbohydrazide-silver proteinate to stain selectively the glycogen content of the glial population. Gliophilic neuronal migration was confirmed in the human fetus, with radial glial fibers (RGF) acting as obligatory corridors for neuronal migration in the prospective neocortex and underlying intermediate zone (IZ). With this method, the entire glial phase was differentiated from neuronal elements; this permitted a description of the evolutinary distribution pattern of RGF: in the cortical plate, glial fascicles fully dissociate by 18 weeks gestation, whereas in the IZ, they remain grouped in fascicles until their transformation into astrocytes. The most conspicuous and constant development feature observed in the maturing glial cytoplasm between 21 and 30 weeks gestation was a radical enhancement in the abundance and activity of the lysosomal apparatus and autophagic vacuoles observed in the RGF, a cytological basis for the transformation of radial glial cells into astrocytes. These data have implications for the understanding of the ontogenesis of the neocortical vertical modules in the human brain and for the phylogenetic analysis of the vertical cortical units in terms of comparative mammalian anatomy.