Metal-linked Immunosorbent Assay (MeLISA): the Enzyme-Free Alternative to ELISA for Biomarker Detection in Serum.
Metal-linked Immunosorbent Assay (MeLISA): the Enzyme-Free Alternative to ELISA for Biomarker Detection in Serum.
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金属联免疫吸附测定 (MeLISA)：用于血清生物标志物检测的 ELISA 的无酶替代方案
DOI：
10.7150/thno.16129
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发表时间：
2016
期刊：
影响因子：
12.4
通讯作者：
Long YT
中科院分区：
文献类型：
--
作者：
Yu RJ;Ma W;Liu XY;Jin HY;Han HX;Wang HY;Tian H;Long YT
临床样品中疾病生物标志物的测定对于疾病监测和公共卫生具有重要意义。主要形式是酶联免疫吸附测定（ELISA），它巧妙地利用了抗原抗体反应和酶的生物催化特性。虽然酶在该平台中发挥重要作用，但与一般化学产品相比，它们通常具有较差的稳定性和对温度、pH条件的耐受性。在这里，我们展示了一种金属联免疫吸附测定（MeLISA）的基础上，一个强大的信号放大机制，忠实地取代酶的基本元素。作为ELISA的无酶替代方法，该方法通过分别检测浓度为0.1 ng mL-1、0.1 ng mL-1和1 ng mL-1的甲胎蛋白（AFP）、前列腺特异性抗原（PSA）和C反应蛋白（CRP）来工作。它的灵敏度比ELISA高2个数量级，显色反应快4倍。AFP和PSA的检测分别被来自肝细胞癌（HCC）和前列腺癌患者的100多份血清样本进一步证实。
Determination of disease biomarkers in clinical samples is of crucial significance for disease monitoring and public health. The dominating format is enzyme-linked immunosorbent assay (ELISA), which subtly exploits both the antigen-antibody reaction and biocatalytic property of enzymes. Although enzymes play an important role in this platform, they generally suffer from inferior stability and less tolerant of temperature, pH condition compared with general chemical product. Here, we demonstrate a metal-linked immunosorbent assay (MeLISA) based on a robust signal amplification mechanism that faithfully replaces the essential element of the enzyme. As an enzyme-free alternative to ELISA, this methodology works by the detection of α-fetoprotein (AFP), prostatic specific antigen (PSA) and C-reactive protein (CRP) at concentrations of 0.1 ng mL-1, 0.1 ng mL-1 and 1 ng mL-1 respectively. It exhibits approximately two magnitudes higher sensitivity and is 4 times faster for chromogenic reaction than ELISA. The detection of AFP and PSA was further confirmed by over a hundred serum samples from hepatocellular carcinoma (HCC) and prostate cancer patients respectively.