Small proteins modulate ion channel-like ACD6 to regulate immunity in Arabidopsis thaliana
Small proteins modulate ion channel-like ACD6 to regulate immunity in Arabidopsis thaliana
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DOI：
10.1101/2021.01.25.428077
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发表时间：
2021-01
期刊：
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通讯作者：
Junbin Chen;Lei Li;Jong Hum Kim;B. Neuhäuser;Mingyu Wang;M. Thelen;Richard Hilleary;Yuan Chi;Luyang Wei;Kavita Venkataramani;Moisés Expósito-Alonso;Chang Liu;J. Keck;A. C. Barragan;R. Schwab;Ulrich Lutz;Zhen-Ming Pei;S. He;U. Ludewig;D. Weigel;Wangsheng Zhu
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作者：
Junbin Chen;Lei Li;Jong Hum Kim;B. Neuhäuser;Mingyu Wang;M. Thelen;Richard Hilleary;Yuan Chi;Luyang Wei;Kavita Venkataramani;Moisés Expósito-Alonso;Chang Liu;J. Keck;A. C. Barragan;R. Schwab;Ulrich Lutz;Zhen-Ming Pei;S. He;U. Ludewig;D. Weigel;Wangsheng Zhu
加速细胞死亡6(ACD6)在拟南芥中调节生长和防御之间的权衡。然而，ACD6和相关蛋白质在植物中作用的确切生化机制仍然是个谜。在这里，我们发现了两个编码∼7 kDa蛋白的基因座，即高活性ACD61的调节器(MHA1)及其类似的MHA1(MHA1L)，它们与特定的ACD6变异体有差异地相互作用。MHA1L促进了ACD6复合体的积累，从而增加了ACD6标准等位基因的活性，以调节植物的生长和防御。ACD6是一种多通道跨膜蛋白，具有与哺乳动物离子通道结构相似的细胞内锚蛋白重复序列。有几条证据表明，ACD6活性的增加与钙内流的增加有关，这可能是由ACD6本身介导的，MHA1L作为ACD6的直接调节因子。
ACCELERATED CELL DEATH 6 (ACD6) mediates a trade-off between growth and defense in Arabidopsis thaliana. However, the precise biochemical mechanism by which ACD6 and related proteins in plants act remains enigmatic. Here, we identified two loci, MODULATOR OF HYPERACTIVE ACD6 1 (MHA1) and its paralog MHA1-LIKE (MHA1L), that code for ∼7 kDa proteins that differentially interact with specific ACD6 variants. MHA1L enhances accumulation of an ACD6 complex, thereby increasing activity of the ACD6 standard allele for regulating plant growth and defenses. ACD6 is a multipass transmembrane protein with intracellular ankyrin repeats that are structurally similar to those found in mammalian ion channels. Several lines of evidence link increased ACD6 activity to enhanced calcium influx, likely mediated by ACD6 itself and with MHA1L as a direct regulator of ACD6.