Influence of the farming system and vine variety on yeast communities associated with grape berries
Influence of the farming system and vine variety on yeast communities associated with grape berries
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DOI：
10.1016/j.ijfoodmicro.2010.11.040
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发表时间：
2011-01-31
影响因子：
5.4
通讯作者：
Valero, Eva
中科院分区：
文献类型：
--
作者：
Cordero-Bueso, Gustavo;Arroyo, Teresa;Valero, Eva
大多数国家的葡萄酒生产都是基于商业菌株的使用，导致这些菌株在酿酒厂中定植，从而减少了本地生物多样性。这意味着葡萄酒风格因此可以标准化。葡萄园可能是酿酒学意义上的本土酵母的重要来源。出于这个原因，本研究的目的是比较两种农艺条件，目的是保持葡萄园中的酵母生物多样性。设计了一个为期三年的采样计划，以评估不同农艺参数对发酵葡萄酵母生物多样性的影响。因此，两个葡萄园，一个有机和一个传统的，有三个不同的葡萄品种（设拉子。Grenache和Barbera）被选中。从两个葡萄园总共收集了27个样本。其中，分离出1080个菌落，共鉴定出9种。通过微卫星分析获得9种不同的电泳图谱，对鉴定为酿酒酵母的菌株进行基因分型。采用经典生态学指标计算了各物种的丰富度（S）、多样性（H '）和优势度（D）。结果表明，在葡萄园中使用的植物检疫处理对葡萄相关酵母的多样性有明显的影响。这是第一次，经典的生态学指标已被用于研究的自然发酵的葡萄须和种念珠菌sorbosa和毕赤酵母toletana已被描述在葡萄园的马德里葡萄酒产区的生态。(c)2010 Elsevier B.V.保留所有权利。
Wine production in most countries is based on the use of commercial strains leading to the colonisation of the wineries by these strains with the consequent reduction of autochthonous biodiversity. This implies that wine styles could therefore become standardised. The vineyard could be an important source of native yeasts of oenological interest. For this reason the objective of this study was to compare two agronomic conditions with the aim of preserving yeast biodiversity in the vineyard. A three year sampling plan was designed to evaluate the influence of different agronomic parameters on the biodiversity of fermentative grape yeasts. Thus two vineyards, one organic and one conventional, with three different grape varieties (Shiraz. Grenache and Barbera) were chosen. In total, 27 samples were collected from both vineyards. Of these, 1080 colonies were isolated and a total of 9 species were identified. The strains identified as Saccharomyces cerevisiae were genotyped by microsatellite analysis obtaining nine different electrophoretic patterns. Classical ecology indexes were used to obtain the richness (S), the biodiversity (H') and the dominance (D) of the species studied. The results indicated a clear influence on grape associated yeast diversity of the phytosanitary treatment used in the vineyard. This is the first time that classical ecology indexes have been used to study the ecology of the spontaneous fermentation of grape musts and the species Candida sorbosa and Pichia toletana have been described in vineyards of the Madrid winegrowing region. (c) 2010 Elsevier B.V. All rights reserved.