Integration site for streptomyces phage φBT1 and development of site-specific integrating vectors
Integration site for streptomyces phage φBT1 and development of site-specific integrating vectors
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DOI：
10.1128/jb.185.17.5320-5323.2003
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发表时间：
2003-09-01
影响因子：
3.2
通讯作者：
Smith, MCM
中科院分区：
文献类型：
--
作者：
Gregory, MA;Till, R;Smith, MCM
尽管温带链霉菌phiC 31和phiBT 1的基因组之间存在广泛的相似性，但attP-int位点保守性很差。在这里，我们证明了phiBT 1整合到一个不同的附着位点比phiC 31。phiBT 1 attB位于SCO 4848内，编码79个氨基酸的推定的整合膜蛋白。基于phiBT 1整合酶的整合载体显示出具有广泛的宿主范围，并且与基于phiC 31 attP-int基因座的整合载体完全相容。
Despite extensive similarities between the genomes of the Streptomyces temperate phages phiC31 and phiBT1, the attP-int loci are poorly conserved. Here we demonstrate that phiBT1 integrates into a different attachment site than phiC31. phiBT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane protein. Integration vectors based on phiBT1 integrase were shown to have a broad host range and are fully compatible with those based on the phiC31 attP-int locus.