Enzyme-Triggered Fluorescence Turn-on: A Probe for Specifically Imaging Ovarian-Cancer-Related γ-Glutamyltranspeptidase
Enzyme-Triggered Fluorescence Turn-on: A Probe for Specifically Imaging Ovarian-Cancer-Related γ-Glutamyltranspeptidase
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酶触发荧光开启：用于特异性成像卵巢癌相关γ-谷氨酰转肽酶的探针
DOI：
10.1002/cjoc.201700248
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发表时间：
2017
影响因子：
5.4
通讯作者：
Zhao Chunchang
中科院分区：
文献类型：
--
作者：
Tian Jie;Yan Qinglong;Zhu Ying;Zhang Jichao;Li Jiao;Shi Ben;Xu Ge;Fan Chunhai;Zhao Chunchang
设计并合成了一种以γ-谷氨酰转肽酶为底物、硼-二吡咯亚甲基为发色团的荧光开启探针。GGT-催化了γ-谷氨酸键的断裂，并在BODIPY的硫和氮原子之间发生芳烃转移，导致了明显的光学变化。这种特异的响应性提供了一种易于区分的荧光信号，可以直观地显示活细胞中的GGT活性，并区分GGT阳性癌细胞和GGT阴性细胞。
A fluorescent turn‐on probe for specifically targetingγ‐glutamyltranspeptidase (GGT) was designed and synthesized by integrating boron‐dipyrromethene (BODIPY) as a chromophore and glutathione (GSH) as the GGT substrate. GGT‐catalyzed the cleavage of theγ‐glutamyl bond and generated the aromatic hydrocarbon transfer between the sulfur and the nitrogen atom in BODIPY, leading to distinct optical changes. Such specific responsiveness provides an easily distinguishable fluorescence signal to visualize the GGT activity in living cells and differentiate GGT‐positive cancer cells from GGT‐negative cells.