Enhanced nasal cytokine production in human beings after in vivo challenge with diesel exhaust particles
Enhanced nasal cytokine production in human beings after in vivo challenge with diesel exhaust particles
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DOI：
10.1016/s0091-6749(96)70233-6
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发表时间：
1996-07-01
影响因子：
14.2
通讯作者：
Saxon, A
中科院分区：
文献类型：
--
作者：
DiazSanchez, D;Tsien, A;Saxon, A
背景：在过去的一个世纪里，柴油废气颗粒(DEP)被认为与全球过敏性呼吸道疾病发病率的增加有关。目的：探讨DEPS对鼻腔粘膜细胞产生细胞因子的影响。方法：用生理盐水或DEPS鼻腔刺激18h后，观察鼻腔灌洗细胞中细胞因子信使RNA的表达水平，以及鼻腔灌洗细胞中可检测到细胞因子mRNA的人数。结果：激发前，大多数受试者鼻腔灌洗细胞仅检测到干扰素-γ、IL-2和IL-13mRNA的表达。刺激后，细胞产生IL-2、IL-4、IL-5、IL-6、IL-10、IL-13和干扰素-γ的mRNA。此外，所有细胞因子的mRNA水平均升高。增强的IL-4蛋白也存在于激发后灌洗液中。虽然激发前后鼻腔灌洗液中的细胞在数量和类型上不一定代表相同的细胞，但细胞因子的广泛增加并不是灌洗液中回收的T细胞增加的结果。结论：暴露于EDPS后鼻腔细胞因子的表达增加可以预测到局部IgE产生的增加，从而在呼吸道变态反应性疾病的发病率增加中发挥作用。
Background: Diesel exhaust particles (DEPs) have been implicated in the worldwide increased incidence of allergic airway disorders over the past century. They can enhance in vivo IgE production in the human upper respiratory mucosa.Objective: The study was carried out to determine whether DEPs can alter the production of cytokines by cells residing in the nasal mucosa.Methods: Eighteen hours after intranasal challenge with saline solution or DEPs, we studied the levels of messenger RNA for cytokines in nasal lavage cells and the number of subjects in whom cytokine mRNA could be detected.Results: Before challenge, most subjects' nasal lavage cells had detectable levels of only interferon-gamma, IL-2, and IL-13 mRNA. After challenge, the cells produced readily detectable mRNA for IL-2, IL-4, IL-5, IL-6, IL-10, IL-13, and interferon-gamma. In addition, the levels of all cytokine mRNA increased. Enhanced IL-4 protein was also present in the postchallenge lavage fluid. Although the cells in nasal lavage before and after challenge do not necessarily represent the same cells either in number or type, the broad increase in cytokine production was not simply the result of an increase in T cells recovered in the lavage fluid.Conclusion: An increase in nasal cytokine expression after exposure to EDPs can be predicted to contribute to enhanced local IgE production and thus play a role in the increased incidence of respiratory allergic disease.