Imaging Neuronal Activity in the Optic Tectum of Late Stage Larval Zebrafish.
Imaging Neuronal Activity in the Optic Tectum of Late Stage Larval Zebrafish.
复制标题
DOI：
10.3390/jdb6010006
复制
发表时间：
2018-03-09
影响因子：
2.7
通讯作者：
Nikolaev A
中科院分区：
文献类型：
--
作者：
Bergmann K;Meza Santoscoy P;Lygdas K;Nikolaeva Y;MacDonald RB;Cunliffe VT;Nikolaev A
斑马鱼是一种成熟的模型来研究体内视觉神经元回路的发育和功能，这主要是由于它们在胚胎和幼虫阶段的光学可及性。在过去的十年中，已经开发了多种实验范式来研究视觉驱动的行为，特别是那些由视顶盖（低级脊椎动物的主要视觉中心）调节的行为。除了少数例外，这些技术仅限于幼幼虫（受精后7-9天，dpf）。然而，许多形式的视觉驱动的行为，如浅滩，出现在后来的发展阶段。因此，需要一种实验范式来成像超过9 dpf的斑马鱼幼鱼的视觉系统。在这里，我们表明，使用NBT：GCaMP 3线允许成像神经元活动的视顶盖在晚期幼虫，直到至少21 dpf。利用这条线，我们已经确定了2-3周龄的鱼在细胞体和神经元的顶盖神经元的感受野特性。NBT：GCaMP 3系提供了一种补充方法和额外的机会来研究晚期斑马鱼幼虫的神经元活动。
The zebrafish is an established model to study the development and function of visual neuronal circuits in vivo, largely due to their optical accessibility at embryonic and larval stages. In the past decade multiple experimental paradigms have been developed to study visually-driven behaviours, particularly those regulated by the optic tectum, the main visual centre in lower vertebrates. With few exceptions these techniques are limited to young larvae (7–9 days post-fertilisation, dpf). However, many forms of visually-driven behaviour, such as shoaling, emerge at later developmental stages. Consequently, there is a need for an experimental paradigm to image the visual system in zebrafish larvae beyond 9 dpf. Here, we show that using NBT:GCaMP3 line allows for imaging neuronal activity in the optic tectum in late stage larvae until at least 21 dpf. Utilising this line, we have characterised the receptive field properties of tectal neurons of the 2–3 weeks old fish in the cell bodies and the neuropil. The NBT:GCaMP3 line provides a complementary approach and additional opportunities to study neuronal activity in late stage zebrafish larvae.