Long noncoding RNA expression profile of mouse cementoblasts under compressive force
Long noncoding RNA expression profile of mouse cementoblasts under compressive force
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压力下小鼠成牙骨质细胞的长非编码RNA表达谱
DOI：
10.2319/061118-438.1
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发表时间：
2019-05-01
影响因子：
3.4
通讯作者：
Li, Weiran
中科院分区：
文献类型：
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作者：
Liu, Hao;Huang, Yiping;Li, Weiran
目的：研究挤压作用下成水泥细胞长链非编码RNA （lncRNA）的表达谱。材料与方法：小鼠成水泥细胞受压（1.5 g/cm(2)） 8小时。采用RNA测序（RNA-seq）比较压缩细胞和对照细胞的转录组。使用实时定量聚合酶链反应（qRT-PCR）验证5个感兴趣的差异表达lncrna。基因本体（GO）功能注释和京都基因与基因组百科全书（KEGG）通路分析。结果：在受压载荷下，成水泥细胞中共有70个lncrna和521个mrna受到差异调控。在差异表达的lncrna中，57个表达上调，13个表达下调。选择的5个lncrna （Prkcz2、Hklos、Trp53cor1、Gdap10和ak312ps）的表达水平通过qRT-PCR验证，与RNA-seq结果一致。氧化石墨烯功能注释表明，在压缩负荷期间，与细胞对缺氧和凋亡过程的反应相关的基因上调。KEGG分析确定了包括缺氧诱导因子-1 α、叉头盒O和哺乳动物雷帕霉素信号通路的关键途径。结论：机械压缩改变了成骨水泥细胞的lncRNA表达谱，为进一步研究lncRNA在正畸治疗中压缩成骨水泥细胞和牙根吸收中的作用和调控提供了重要参考。
Objectives: To investigate the long noncoding RNA (lncRNA) expression profile of cementoblasts under compressive force.Materials and Methods: Mouse cementoblasts were exposed to compression (1.5 g/cm(2)) for 8 hours. RNA sequencing (RNA-seq) was performed to compare the transcriptomes of the compressed and control cells. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate five of the differentially expressed lncRNAs of interest. Gene Ontology (GO) functional annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were also performed.Results: A total of 70 lncRNAs and 521 mRNAs were differentially regulated in cementoblasts subjected to compressive loading. Among the differentially expressed lncRNAs, 57 were upregulated and 13 downregulated. The expression levels of the five selected lncRNAs (Prkcz2, Hklos, Trp53cor1, Gdap10, and Ak312-ps) were validated by qRT-PCR and consistent with the RNA-seq results. GO functional annotation demonstrated upregulation of genes associated with cellular response to hypoxia and apoptotic processes during compressive loading. KEGG analysis identified the crucial pathways involving the hypoxia-inducing factor-1 alpha, forkhead box O, and mammalian target of rapamycin signaling pathways.Conclusions: Mechanical compression changes the lncRNA expression profile of cementoblasts, providing important references for further investigation into the role and regulation of lncRNAs in compressed cementoblasts and root resorption during orthodontic treatment.