MECHANISM OF CROSSLINKING OF PROTEINS BY GLUTARALDEHYDE .2. REACTION WITH MONOMERIC AND POLYMERIC COLLAGEN
MECHANISM OF CROSSLINKING OF PROTEINS BY GLUTARALDEHYDE .2. REACTION WITH MONOMERIC AND POLYMERIC COLLAGEN
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DOI：
10.3109/03008208209034419
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发表时间：
1982-01-01
影响因子：
2.9
通讯作者：
NIMNI, ME
中科院分区：
文献类型：
--
作者：
CHEUNG, DT;NIMNI, ME
不同浓度的戊二醛作用于天然可溶分子、变性分子和重组纤维3种不同状态的胶原。通过测量胶原蛋白的溶解度β来评估分子内和分子间的交联度。和伽马。链的形成和对CNBr或胶原酶消化的裂解的抵抗。通过氨基酸分析测定赖氨酰残基的变性程度。当稀胶原溶液与低浓度的戊二醛反应时，观察到分子内交联物是主要的交联物。随着戊二醛浓度的增加，胶原蛋白变得更难溶，表明形成了分子间的交联剂。当重组的胶原纤维与低浓度的戊二醛反应时，会形成分子间的交联剂，从而防止材料被CNBr2溶解。这些材料仍然可以被胶原酶溶解。随着戊二醛浓度的增加，材料对胶原酶的抗性增强，而赖氨酸残基的修饰数量仅略有增加。这反映了从最初的戊二醛和赖氨酸残基反应链延伸出来的戊二醛聚合物的分子长度的增加，而不是实际交联点数量的增加。
Collagen in 3 different states, i.e., native soluble molecules, denatured molecules and reconstituted fibers, was exposed to various concentrations of glutaraldehyde. The degree of intramolecular and intermolecular crosslink formation was evaluated by measuring collagen solubility, .beta. and .gamma. chain formation and resistance towards cleavage by CNBr or collagenase digestion. Modification of lysyl residues was measured by amino acid analysis. When dilute collagen solutions were reacted with low concentrations of glutaraldehyde, intramolecular crosslinks were observed as the predominant crosslinks. When the glutaraldehyde concentration was increased, the collagen became more insoluble, indicating the formation of intermolecular crosslinks. When reconstituted collagen fibers were reacted with low concentrations of glutaraldehyde, intermolecular crosslinks were formed, which prevented the material from being solubilized by CNBr. These materials could still be solubilized by collagenase. When the glutaraldehyde concentration was increased, the materials became resistant to collagenase, while there was only a small increase in the number of lysyl residues modified. This reflects an increase in the molecular length of the glutaraldehyde polymers extending from the initial glutaraldehyde and lysyl residue reaction sties rather than an increase in the actual number of crosslinking sites.