Bacterial Site-Specific DNA Inversion Systems
Bacterial Site-Specific DNA Inversion Systems
复制标题
细菌位点特异性 DNA 反转系统
DOI：
--
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Reid C. Johnson
中科院分区：
文献类型：
--
作者：
Reid C. Johnson
涉及DNA倒位的专门重组反应已经进化为在种群内产生遗传多样性的机制。它们的作用往往是使一部分人口预先适应环境的突然变化，或使一部分人口能够利用新的情况。位点特异性DNA倒位反应的特征在于在限定位点发生的重组事件，并且通常由专用于该特定反应的酶催化。低转化率被认为主要受到细胞中存在的极少量Hin蛋白的限制，因为过表达Hin协调地增加转化率。体内研究表明，整合宿主因子（IHF）和亮氨酸应答调节蛋白（LRP）参与了膜反转反应。这两种DNA弯曲蛋白被认为一起起作用，以帮助促进IRL和IRR之间的突触。在lrp突变体中，FimB和FimE促进的转化率也降低。LRP是一种中等丰度的DNA结合和弯曲蛋白，其作为参与氨基酸代谢和菌毛生物合成的各种基因的全局调节剂发挥作用。由Hin和Gin DNA转化酶催化的水解已经在体外被深入研究，并且该反应的基本轮廓被很好地理解。DNA反转发生在简单的缓冲盐溶液中，除了需要负超螺旋底物之外，不需要高能辅因子。
Specialized recombination reactions involving DNA inversions have evolved as a mechanism to generate genetic diversity within a population. They often function to preadapt a portion of a population to a sudden change in the environment or to allow a portion of a population to take advantage of a new situation. Site-specific DNA inversion reactions are characterized by recombination events which occur at defined sites and are usually catalyzed by an enzyme dedicated to that particular reaction. The low rate of inversion is believed to be primarily limited by the extremely low amounts of the Hin protein present in cells since overexpressing Hin coordinately increases inversion rates. In vivo studies have demonstrated that integration host factor (IHF) and leucine-responsive regulatory protein (LRP) participate in the fim inversion reaction. These two DNA bending proteins are believed to function together to help promote synapsis between IRL and IRR. FimB- and FimE-promoted inversion rates are also decreased in lrp mutants. LRP is a moderately abundant DNA binding and bending protein that functions as a global regulator of various genes involved in amino acid metabolism and fimbria biosynthesis. Recombination catalyzed by the Hin and Gin DNA invertases has been intensively studied in vitro, and the basic outline of the reaction is well understood. DNA inversion occurs in a simple buffered salt solution without the need for a high-energy cofactor other than the requirement for a negatively supercoiled substrate.
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影响因子：
10.5
作者：
PAULL, TT;HAYKINSON, MJ;JOHNSON, RC
通讯作者：
JOHNSON, RC
沙门氏菌的相变：体内 Hin 重组酶和 hix 重组位点相互作用的分析。
影响因子：
10.5
作者：
Hughes,KT;Youderian,P;Simon,MI
通讯作者：
Simon,MI
通过与 1,10-菲咯啉铜 (I) 复合物缀合的蛋白质探测大肠杆菌 Fis-DNA 复合物的结构。
DOI：
10.1073/pnas.91.5.1721
发表时间：
1994
影响因子：
11.1
作者：
Pan,CQ;Feng,JA;Finkel,SE;Landgraf,R;Sigman,D;Johnson,RC
通讯作者：
Johnson,RC
DOI：
10.1016/s0021-9258(18)63741-x
发表时间：
1989-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Yvonne Hodges-Garcia;P. Hagerman;D. Pettijohn
通讯作者：
Yvonne Hodges-Garcia;P. Hagerman;D. Pettijohn
影响因子：
11.4
作者：
ROSS, W;THOMPSON, JF;GOURSE, RL
通讯作者：
GOURSE, RL