Impact of Longevity Interventions on a Validated Mouse Clinical Frailty Index
Impact of Longevity Interventions on a Validated Mouse Clinical Frailty Index
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长寿干预对经验证的小鼠临床虚弱指数的影响
DOI：
10.1093/gerona/glu315
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发表时间：
2016-03-01
影响因子：
5.1
通讯作者：
Mitchell, Sarah J.
中科院分区：
文献类型：
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作者：
Kane, Alice E.;Hilmer, Sarah N.;Mitchell, Sarah J.
本文研究了已知影响小鼠寿命和健康寿命的因素对小鼠虚弱指数（FI）的影响：品系（短寿命DBA/2 J小鼠与长寿命C57 BL/6 J小鼠），卡路里限制（CR）和白藜芦醇治疗。基于缺陷累积的小鼠FI最近由Whitehead JC，Hildebrand BA，Sun M等人在C57 BL/6 J小鼠中验证（A clinical frailty index in aging mice：comparison with frailty index data in humans.老年人生物科学与医学杂志. 2014;69：621-632），并且与人FI具有许多特征。在自由采食和CR DBA/2 J和C57 BL/6 J小鼠中测量雄性和雌性年龄（18个月）的FI评分，以及在饮食中自由采食或不含白藜芦醇（100 mg/kg/天）的雄性年龄（24个月）的C57 BL/6 J小鼠6个月。使用两名评定者的平均评分，评定者具有良好的评定者间可靠性（ICC = 0.88，95% CI [0.80，0.92]）。此外，与年龄匹配的对照组相比，CR和白藜芦醇的干预与C57 BL/6 J小鼠的FI评分显著降低相关。对于雄性热量限制组，短寿命DBA/2 J小鼠的FI评分也略高于C57 BL/6 J小鼠（DBA/2 J FI = 0.16 +/- 0.03，C57 BL/6 J FI = 0.11 +/- 0.03，p = 0.01）。本研究使用由Whitehead JC、Hildebrand BA、Sun M等人开发的小鼠FI（A clinical frailty index in aging mice：comparison with frailty index data in humans.老年学生物科学医学科学杂志二〇一四年69：621-632），并显示该工具可用于量化饮食和药物干预对虚弱的影响。
This article investigates the effect on the mouse frailty index (FI), of factors known to influence lifespan and healthspan in mice: strain (short-lived DBA/2J mice vs long-lived C57BL/6J mice), calorie restriction (CR), and resveratrol treatment. The mouse FI, based on deficit accumulation, was recently validated in C57BL/6J mice by Whitehead JC, Hildebrand BA, Sun M, et al. (A clinical frailty index in aging mice: comparisons with frailty index data in humans. J Gerontol A Biol Sci Med Sci. 2014;69:621-632) and shares many characteristics of the human FI. FI scores were measured in male and female aged (18 months) ad-libitum fed and CR DBA/2J and C57BL/6J mice, as well as male aged (24 months) C57BL/6J mice ad-libitum fed with or without resveratrol (100 mg/kg/day) in the diet for 6 months. Mean scores of two raters were used, and the raters had excellent inter-rater reliability (ICC = 0.88, 95% CI [0.80, 0.92]). Furthermore, the interventions of CR and resveratrol were associated with a significant reduction in FI scores in C57BL/6J mice, compared to age-matched controls. The short-lived DBA/2J mice also had slightly higher FI scores than the C57BL/6J mice, for the male calorie-restricted groups (DBA/2J FI = 0.16 +/- 0.03, C57BL/6J FI = 0.11 +/- 0.03, p = .01). This study uses the mouse FI developed by Whitehead JC, Hildebrand BA, Sun M, et al. (A clinical frailty index in aging mice: comparisons with frailty index data in humans. J Gerontol A Biol Sci Med Sci. 2014;69:621-632) in a different mouse colony and shows that this tool can be applied to quantify the effect of dietary and pharmaceutical interventions on frailty.