Propagation of differentiating normal human tracheobronchial epithelial cells in serum-free medium.
Propagation of differentiating normal human tracheobronchial epithelial cells in serum-free medium.
复制标题
在无血清培养基中分化正常人气管支气管上皮细胞的增殖。
DOI：
10.1002/jcp.1041300202
复制
发表时间：
1987
影响因子：
5.6
通讯作者：
Siddiqui,KM
中科院分区：
文献类型：
--
作者：
Chopra,DP;Sullivan,J;Wille,JJ;Siddiqui,KM
Serial‐passage cultures of normal human tracheobronchial (TB) epithelial cells that exhibit functional differentiation have been established in serum‐free medium supplemented with bovine pituitary extract (25 μg/ml), insulin (5 μg/ml), hydrocortisone (0.5 μg/ml), EGF (5 ng/ml), 10−6M each of ethanolamine and phoshoethanolamine, and antibiotics. The cells proliferated in this medium with a population doubling time of approximately 80 hours. Further, the passaged cultures retained differentiated morphology as evidenced by secretion of glycoproteins, binding of concanavalin A lectin, and presence of alcian blue and periodic acid Schiff‐positive material in their cytoplasm. Ultrastructural observations further supported the functional epithelial nature of the cultures. Most cells exhibited characteristic microvilli on cell surfaces and showed junctional complexes between them. The cytoplasm contained a large number of perinuclear secretory vesicles, a characteristic feature of the differentiated cells. These cultures provide an excellent model to study factors that regulate synthesis and secretion of glycoproteins in normal human TB cells.
