A Novel Effector Protein, MJ-NULG1a, Targeted to Giant Cell Nuclei Plays a Role in Meloidogyne javanica Parasitism
A Novel Effector Protein, MJ-NULG1a, Targeted to Giant Cell Nuclei Plays a Role in Meloidogyne javanica Parasitism
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一种针对巨细胞核的新型效应蛋白 MJ-NULG1a 在爪哇根结线虫寄生中发挥作用
DOI：
10.1094/mpmi-05-12-0114-fi
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发表时间：
2013-01-01
影响因子：
3.5
通讯作者：
Liao, Jinling
中科院分区：
文献类型：
--
作者：
Lin, Borong;Zhuo, Kan;Liao, Jinling
分泌效应蛋白在根结线虫食道腺中的表达被认为在线虫入侵寄主根系和线虫完成其生活史所必需的取食位点的形成中起关键作用。本研究通过抑制性消减杂交技术分离到一个在爪哇根结线虫背腺中特异表达的效应蛋白基因Mj-nulg 1a。Southern杂交和BLAST搜索分析表明，Mj-nulg 1a是唯一的根结线虫属。实时逆转录聚合酶链反应分析表明，Mj-nulg 1a的表达上调寄生第二阶段的青少年和下降，在以后的寄生阶段。MJ-NULG 1a包含两个推定的核定位信号，并且一致地，在植物免疫定位分析中表明，MJ-NULG 1a在线虫寄生期间定位于巨细胞的核中。在植物体内，针对Mj-nulg 1a的RNA干扰抑制了线虫中Mj-nulg 1a的表达，并减弱了M. javanica。相反，表达Mj-nulg 1a的转基因拟南芥对M. javanica感染比野生型对照植物。这些结果描述了一种新的线虫效应物，其靶向巨细胞核并在M.爪哇寄生
Secretory effector proteins expressed within the esophageal glands of root-knot nematodes (Meloidogyne spp.) are thought to play key roles in nematode invasion of host roots and in formation of feeding sites necessary for nematodes to complete their life cycle. In this study, a novel effector protein gene designated as Mj-nulg1a, which is expressed specifically within the dorsal gland of Meloidogyne javanica, was isolated through suppression subtractive hybridization. Southern blotting and BLAST search analyses showed that Mj-nulg1a is unique for Meloidogyne spp. A real-time reverse-transcriptase polymerase chain reaction assay showed that expression of Mj-nulg1a was upregulated in parasitic second-stage juveniles and declined in later parasitic stages. MJ-NULG1a contains two putative nuclear localization signals and, consistently, in plant immunolocalization analysis showed that MJ-NULG1a was localized in the nuclei of giant cells during nematode parasitism. In planta RNA interference targeting Mj-nulg1a suppressed the expression of Mj-nulg1a in nematodes and attenuated parasitism ability of M. javanica. In contrast, transgenic Arabidopsis expressing Mj-nulg1a became more susceptible to M. javanica infection than wild-type control plants. These results depict a novel nematode effector that is targeted to giant cell nuclei and plays a critical role in M. javanica parasitism.