UV irradiation-induced apoptosis leads to activation of a 36-kDa myelin basic protein kinase in HL-60 cells
UV irradiation-induced apoptosis leads to activation of a 36-kDa myelin basic protein kinase in HL-60 cells
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DOI：
10.1073/pnas.93.17.8977
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发表时间：
1996-08-20
影响因子：
11.1
通讯作者：
Richie, JP
中科院分区：
文献类型：
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作者：
Lu, ML;Sato, M;Richie, JP
紫外线照射在3小时内诱导HL - 60早幼粒细胞白血病细胞凋亡（或程序性细胞死亡）。紫外线诱导的凋亡伴随着一种36 - kDa髓鞘碱性蛋白激酶（p36 MBP激酶）的激活。这种激酶也可被冈田酸和视黄酸诱导的凋亡所激活。无论诱导剂如何，p36 MBP激酶的激活仅限于实际发生凋亡的细胞亚群。p36 MBP激酶在去核的胞质体中被激活，这表明在信号传导中不需要细胞核或断裂的DNA。我们还分别利用人前列腺肿瘤细胞系LNCap和NIH 3T3成纤维细胞证明了在肿瘤坏死因子 - α和血清饥饿诱导的细胞死亡中p36激酶的激活。我们推测p36 MBP激酶是导致凋亡的不同信号通路中的一个共同组分。
UV irradiation induces apoptosis (or programmed cell death) in HL-60 promyelocytic leukemia cells within 3 h. UV-induced apoptosis is accompanied by activation of a 36-kDa myelin basic protein kinase (p36 MBP kinase). This kinase is also activated by okadaic acid and retinoic acid-induced apoptosis. Irrespective of the inducing agent, p36 MBP kinase activation is restricted to the sub-population of cells actually undergoing apoptosis, Activation of p36 MBP kinase occurs in enucleated cytoplasts, indicating no requirement for a nucleus or fragmented DNA in signaling, We also demonstrate the activation of p36 kinase in tumor necrosis factor-alpha- and serum starvation-induced cell death using the human prostatic tumor cell line LNCap and NIH 3T3 fibroblasts, respectively, We postulate that p36 MBP kinase is a common component in diverse signaling pathways leading to apoptosis.