Enhanced biological phosphate removal from wastewater and clade-level population dynamics of "Candidatus Accumulibacter phosphatis" under free nitrous acid inhibition: Linked with detoxication
Enhanced biological phosphate removal from wastewater and clade-level population dynamics of "Candidatus Accumulibacter phosphatis" under free nitrous acid inhibition: Linked with detoxication
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在游离亚硝酸抑制下，增强废水中的生物除磷能力和“Candidatus Accumulibacter Phostis”的进化枝水平种群动态：与解毒相关
DOI：
10.1016/j.cej.2016.03.063
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发表时间：
2016
影响因子：
15.1
通讯作者：
Peng Yongzhen
中科院分区：
文献类型：
--
作者：
Zeng Wei;Wang Anqi;Zhang Jie;Zhang Limin;Peng Yongzhen
聚磷菌属是一种优势聚磷菌，可实现废水的强化生物除磷。游离亚硝酸（FNA）作为硝化和反硝化的中间产物，普遍存在于污水处理厂和实验室反应器中。研究了FNA对生活污水处理过程中富集菌EBPR和群落结构的影响。pH 6.5时FNA对好氧吸磷的完全抑制浓度为6.85 × 10− 3 mgHNO 2-N/L，高于pH 7.0时。在完全抑制的情况下，聚-β-羟基脂肪酸酯的降解用于亚硝酸盐还原和解毒，而不是P-吸收。在好氧阶段结束时，磷以复合态磷、无机磷、化学参与态磷和邻位态磷的形式存在，复合态磷的比例较非抑制态有所下降。优势聚菌属为IIC分支，占聚菌属总数的70%。IIF比例的下降表明其对FNA的敏感性。IID百分比的增加表明其对FNA的耐受性较强。在pH 7.0时，接种污泥的Shannon指数从3.45下降到1.92，生物多样性下降。因此，FNA抑制减弱了EBPR的性能，并显着改变了分支水平的群落结构的积累。
“CandidatusAccumulibacter” are the dominant polyphosphate-accumulating organism (PAOs) to perform enhanced biological phosphate removal (EBPR) from wastewater. Free nitrous acid (FNA) as inhibitor is an intermediate of nitrification and denitrification commonly present in wastewater treatment plants (WWTPs) and lab-scale reactors. Impact of FNA on EBPR and community structures of Accumulibacter in domestic wastewater treatment was investigated. FNA complete inhibit concentration on aerobic P uptake at pH 6.5 was 6.85 × 10−3mg HNO2-N/L, higher than that at pH 7.0. Under complete inhibition, poly-β-hydroxyalkanoates degradation was used for nitrite reduction and detoxication rather than P-uptake. The P fraction existed in the forms of complex-P, interstitial-P, chemical participated-P and ortho-P at the end of aerobic phase, and complex-P proportion declined in comparison to non-inhibition. Dominant Accumulibacter clade was IIC, accounting for 70% of total Accumulibacter. Decline of IIF proportion indicated its sensitivity to FNA. Increase of IID percentage implied its strong tolerance to FNA. Under inhibition, Shannon index decreased from 3.45 of seed sludge to 1.92 at pH 7.0, indicating decline of biodiversity. Therefore, FNA inhibition weakened EBPR performance, and remarkably changed clade-level community structures of Accumulibacter.