Calreticulin enriched as an early-stage encapsulation protein in wax moth Galleria mellonella larvae
Calreticulin enriched as an early-stage encapsulation protein in wax moth Galleria mellonella larvae
复制标题
DOI：
10.1016/s0145-305x(01)00081-7
复制
发表时间：
2002-05-01
影响因子：
2.9
通讯作者：
Lee, BL
中科院分区：
文献类型：
--
作者：
Choi, JY;Whitten, MMA;Lee, BL
为了研究昆虫早期包埋反应的分子机制，我们从注射的珠子中提纯了一种47 kDa的蛋白，并将其注入到梅氏鸡尾藻幼虫中。克隆出的47 kDa蛋白与果蝇和人钙网织蛋白有很高的同源性。Western blotting分析表明，47 kDa蛋白存在于血细胞中，但不存在于血浆中。用47 kDa蛋白抗体包被早期胶囊化小球，再注射入梅隆螺旋体幼虫体内，可抑制进一步的胶囊化反应。这些结果表明，钙网蛋白参与了无脊椎动物细胞防御反应中的非自我识别。(C)2002爱思唯尔科学有限公司。保留所有权利。
To investigate the molecular mechanism of the early-stage encapsulation reaction in insects, we purified a 47 kDa protein from injected beads into Galleria mellonella larvae. When a cDNA clone was isolated, the 47 kDa protein showed high homology with Drosophila and human calreticulin. Western blotting analysis showed that the 47 kDa protein was present in the hemocytes, but not in the plasma. When the early-stage encapsulated beads were coated with 47 kDa protein antibody and reinjected into G. mellonella larvae, any further encapsulation reaction was inhibited. These results suggest that calreticulin is involved in non-self recognition in invertebrate cellular defense reactions. (C) 2002 Elsevier Science Ltd. All rights reserved.