Synaptic development of the mouse dorsal lateral geniculate nucleus.
Synaptic development of the mouse dorsal lateral geniculate nucleus.
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DOI：
10.1002/cne.22223
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发表时间：
2010-03-01
期刊：
影响因子：
--
通讯作者：
Guido W
中科院分区：
文献类型：
--
作者：
Bickford ME;Slusarczyk A;Dilger EK;Krahe TE;Kucuk C;Guido W
小鼠外侧膝状体背侧核（dLGN）已成为研究丘脑回路发育的模型系统。然而，仍然缺乏关于各种类型的视网膜和非视网膜突触如何以及何时发育的信息。我们研究了共同色素C57/BL 6株的发育小鼠dLGN的突触组织，通过记录电刺激视束轴突引起的突触反应，并通过研究识别的突触的超微结构。在出生后早期（<P12），视束诱发反应主要是兴奋性的。直到睁眼后（>P14）才出现抑制反应的完整补充，此时视束刺激通常诱发兴奋性突触后电位/抑制性突触后电位（EPSP/IPSP）序列，后者具有GABAA和GABAB成分。电生理和超微结构观察结果一致。在P7，存在许多突触，但突触轮廓缺乏成年dLGN的超微结构特征，并且通过使用免疫细胞化学技术可以检测到很少的γ-氨基丁酸（GABA）。与此相反，P14，GABA染色是强大的，成熟的视网膜和非视网膜起源的突触配置文件很容易区分，突触接触的大小和比例与成人相似。非视网膜突触的出现与视网膜小神经连接的修剪以及视网膜活动从自发到视觉驱动的转变相一致。这些结果表明，小鼠dLGN的突触结构是类似于其他高等哺乳动物，从而提供了进一步的支持，其作为视觉系统发育的模型系统。
The dorsal lateral geniculate nucleus (dLGN) of the mouse has emerged as a model system in the study of thalamic circuit development. However, there is still a lack of information regarding how and when various types of retinal and nonretinal synapses develop. We examined the synaptic organization of the developing mouse dLGN in the common pigmented C57/BL6 strain, by recording the synaptic responses evoked by electrical stimulation of optic tract axons, and by investigating the ultrastructure of identified synapses. At early postnatal ages (<P12), optic tract evoked responses were primarily excitatory. The full complement of inhibitory responses did not emerge until after eye opening (>P14), when optic tract stimulation routinely evoked an excitatory postsynaptic potential/inhibitory postsynaptic potential (EPSP/IPSP) sequence, with the latter having both a GABAA and GABAB component. Electrophysiological and ultrastructural observations were consistent. At P7, many synapses were present, but synaptic profiles lacked the ultrastructural features characteristic of the adult dLGN, and little γ-aminobutyric acid (GABA) could be detected by using immunocytochemical techniques. In contrast, by P14, GABA staining was robust, mature synaptic profiles of retinal and nonretinal origin were easily distinguished, and the size and proportion of synaptic contacts were similar to those of the adult. The emergence of nonretinal synapses coincides with pruning of retinogeniculate connections, and the transition of retinal activity from spontaneous to visually driven. These results indicate that the synaptic architecture of the mouse dLGN is similar to that of other higher mammals, and thus provides further support for its use as a model system for visual system development.
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影响因子：
2.5
作者：
FAMIGLIETTI, EV;PETERS, A
通讯作者：
PETERS, A
影响因子：
2.5
作者：
GODEMENT, P;SALAUN, J;IMBERT, M
通讯作者：
IMBERT, M
影响因子：
2.5
作者：
CUCCHIARO, JB;UHLRICH, DJ;SHERMAN, SM
通讯作者：
SHERMAN, SM
DOI：
10.1073/pnas.92.24.11145
发表时间：
1995-11-21
影响因子：
11.1
作者：
CLASCA, F;ANGELUCCI, A;SUR, M
通讯作者：
SUR, M
影响因子：
2.5
作者：
HAMOS, JE;VANHORN, SC;SHERMAN, SM
通讯作者：
SHERMAN, SM