Allosteric transitions in biological nanomachines are described by robust normal modes of elastic networks.
Allosteric transitions in biological nanomachines are described by robust normal modes of elastic networks.
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DOI：
10.2174/138920309787847608
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发表时间：
2009-04
影响因子：
2.8
通讯作者：
Thirumalai D
中科院分区：
文献类型：
--
作者：
Zheng W;Brooks BR;Thirumalai D
变构形成了各种酶中分子内通讯的基础，然而潜在的构象变化在很大程度上是难以捉摸的。最近，我们提出了采用弹性模型为基础的正常模式分析，研究变构转换在几个分子纳米机器（包括肌球蛋白II，DNA聚合酶和伴侣蛋白GroEL）。结合生物信息学分析的进化序列的变化，我们已经能够确定高度保守和强大的集体运动模式，能够在长距离传输分子信号。
Allostery forms the basis of intra-molecular communications in various enzymes, however the underlying conformational changes are largely elusive. Recently, we have proposed to employ an elastic model based normal mode analysis to investigate the allosteric transitions in several molecular nanomachines (including myosin II, DNA polymerase and chaperonin GroEL). After combining with bioinformatics analysis of the evolutionary sequence variations, we have been able to identify the highly conserved and robust modes of collective motions that are capable of transmitting molecular signals over long distances.