STUDIES WITH INACTIVATED EQUINE INFLUENZA VACCINE .1. SEROLOGICAL RESPONSES OF PONIES TO GRADED DOSES OF VACCINE
STUDIES WITH INACTIVATED EQUINE INFLUENZA VACCINE .1. SEROLOGICAL RESPONSES OF PONIES TO GRADED DOSES OF VACCINE
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DOI：
10.1017/s0022172400029004
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发表时间：
1983-01-01
影响因子：
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通讯作者：
SCHILD, GC
中科院分区：
文献类型：
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作者：
WOOD, JM;MUMFORD, J;SCHILD, GC
在血清阴性小马中检查了对 3 种不同效力的二价水性马流感疫苗和含有灭活 A/马/布拉格/56 (H7N7) 和 A/马/迈阿密/63 (H3N8) 病毒的佐剂二价疫苗的血清学反应。通过单径向扩散测试测量的疫苗效力范围为每剂量4至56μg血凝素(HA)抗原活性/病毒株。通过血凝抑制（HI）和单径向溶血（SRH）试验检查疫苗接种的血清学反应。初次接种 4 周后，对两种疫苗病毒的 HI 反应几乎检测不到；第二剂后，对 A/Miami/63 病毒的 HI 反应较低或无法检测到，但对 A/Prague/56 病毒的 HI 反应较高（17/20 小马，滴度≥1:16）。 SRH 测试显示，在接种 1 剂和 2 剂疫苗后，两种病毒株的抗体反应均与剂量相关；第二次给药后的水平比初次给药后的水平高 2 至 5 倍。使用含有比最有效的水性疫苗少2至4倍的抗原(13-23μg HA)的佐剂疫苗获得了最高的疫苗接种后抗体滴度。接种后抗体在 SRH 测试中反应良好，是马流感病毒的最新抗原变体。一个值得注意的发现是，在接种 1 或 2 剂疫苗后，通过 HI 或 SRH 测试检测到的抗体下降率很高。即使在加强剂量后 2-4 周内疫苗后抗体水平最高的动物中，抗体水平在 14 周后也下降至较低或不可检测的水平。然而，接种后 14-32 周检测到的低抗体滴度与疫苗剂量相关。
Serological responses to 3 bivalent aqueous equine influenza vaccines of different potency and an adjuvanted bivalent vaccine containing inactivated A/equine/Prague/56 (H7N7) and A/equine/Miami/63 (H3N8) viruses, were examined in seronegative ponies. Potencies of the vaccines, measured by single-radial-diffusion tests, ranged from 4 to 56 .mu.g of hemagglutinin (HA) antigen activity/virus strain per dose. Serological responses to vaccination were examined by hemagglutination-inhibition (HI) and single-radial-hemolysis (SRH) tests. Four weeks after a primary dose, HI responses to both vaccine viruses were barely detectable; after a 2nd dose the HI responses to A/Miami/63 virus were low or undetectable but HI responses to A/Prague/56 virus were higher (17/20 ponies with titers .gtoreq. 1:16). SRH tests revealed dose-related antibody responses to both virus strains after 1 and 2 vaccine doses; levels after the second dose were 2- to 5-fold higher than after the primary dose. Highest post-vaccination antibody titers were obtained with the adjuvanted vaccine which contained 2- to 4-fold less antigen (13-23 .mu.g HA) than the most potent aqueous vaccine. Post-vaccination antibody reacted well in SRH tests were recent antigenic variants of equine influenza virus. A remarkable finding was the high rate of decline in antibody, detected by HI or SRH tests, following 1 or 2 doses of vaccine. Even in animals with the highest post-vaccine antibody levels 2-4 wk after a booster dose, antibody levels had declined to low or undetectable levels 14 wk later. The low antibody titers detected at 14-32 wk after vaccination were nevertheless vaccine dose-related.