Co-localization of choline acetyltransferase and tyrosine hydroxylase within neurons of the dorsal motor nucleus of the vagus.
Co-localization of choline acetyltransferase and tyrosine hydroxylase within neurons of the dorsal motor nucleus of the vagus.
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DOI：
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发表时间：
1990-03
影响因子：
2.8
通讯作者：
D. Armstrong;L. Manley;J. Haycock;L. Hersh
中科院分区：
文献类型：
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作者：
D. Armstrong;L. Manley;J. Haycock;L. Hersh
在本研究中，我们采用了双重免疫荧光标记的程序，以确定生物合成酶酪氨酸羟化酶（TH）和胆碱乙酰转移酶（ChAT）的共同定位在神经元内的背侧延髓。在该区域内，TH标记的神经元分布在孤束核和迷走神经背侧运动核的内侧。多巴胺-β-羟化酶免疫反应性TH标记的细胞内的迷走神经背侧运动核的内侧部分的情况下，表明这些神经元是多巴胺能的。胆碱能胞体也存在于延髓背侧，分布于迷走神经背侧运动核和舌下神经核。在这些ChAT阳性的胞体中，一小部分局限于迷走神经背侧运动核的内侧（即，对应于多巴胺神经元的位置）也含有TH。TH的存在内的ChAT阳性神经元的迷走神经背侧运动核提供了一个解剖学基板，这表明，儿茶酚胺能和胆碱能纤维有助于迷走神经，并可能有助于解释一些心脏和胃的影响，导致全身给药的儿茶酚胺剂。
In the present study, we employed a dual-immunofluorescent labeling procedure to determine if the biosynthetic enzymes tyrosine hydroxylase (TH) and choline acetyltransferase (ChAT) are co-localized within neurons in the dorsal medulla of rat. Within this region TH-labeled neurons are distributed within the nuclei of the solitary tracts and medial aspect of the dorsal motor nucleus of the vagus. The absence of dopamine-beta-hydroxylase immunoreactivity within TH-labeled cells in the medial portion of the dorsal motor nucleus of the vagus suggests that these neurons are dopaminergic. Cholinergic perikarya also are present in the dorsal medulla and are distributed throughout the dorsal motor nucleus of the vagus and hypoglossal nucleus. Of these ChAT-positive perikarya, a small percentage limited to the medial aspect of the dorsal motor nucleus of the vagus (i.e., corresponding to the location of dopamine neurons) also contain TH. The existence of TH within ChAT-positive neurons in the dorsal motor nucleus of the vagus provides an anatomical substrate with which to suggest that catecholaminergic and cholinergic fibers contribute to the vagus nerve and may serve to explain some of the cardiac and gastric effects resulting from systemic administration of catecholamine agents.