Adipogenic potential of adipose stem cell subpopulations.
Adipogenic potential of adipose stem cell subpopulations.
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DOI：
10.1097/prs.0b013e318221db33
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发表时间：
2011-09
影响因子：
3.6
通讯作者：
Rubin JP
中科院分区：
文献类型：
--
作者：
Li H;Zimmerlin L;Marra KG;Donnenberg VS;Donnenberg AD;Rubin JP
脂肪干细胞是一个异质性的群体。了解亚群的功能特征将有助于开发基于脂肪干细胞的再生医学应用疗法。本研究的目的是根据表面标志物表达确定基质血管组分中的不同群体，并评估每种细胞类型分化为成熟脂肪细胞的能力。通过腹壁成形术从人类患者获得皮下全脂肪组织。分离基质血管组分，并通过流式细胞术分离四个细胞群并进行研究。候选血管周细胞（周细胞）定义为CD 146 +/CD 31 −/CD 34 −。检测到两个CD 31+内皮细胞群，并通过CD 34表达进行区分。这些暂时被指定为成熟内皮细胞（CD 31+/CD 34 −）和未成熟内皮细胞（CD 31 +/CD 34+）。两种内皮细胞群在CD 146方面是异质性的。CD 31 −/CD 34+部分（前脂肪细胞候选者）也是CD 90+，但缺乏CD 146表达。CD 31 −/CD 34+组的增殖最大，CD 146+组最慢。在体外成脂分化后，与所有其他人群相比，CD 31 −/CD 34+组的成脂基因、过氧化物酶体增殖物激活受体-γ和脂肪酸结合蛋白4的表达显著更高。该组也显示出最高比例的AdipoRed脂质染色。作者从成人脂肪组织中分离出四种不同的基质群，并表征了它们的成脂潜力。在这四个群体中，CD 31/CD 34+组是最普遍的，并且具有最大的成脂分化潜力。这种细胞类型似乎最有希望用于脂肪组织工程。
Adipose stem cells represent a heterogenous population. Understanding the functional characteristics of subpopulations will be useful in developing adipose stem cell–based therapies for regenerative medicine applications. The aim of this study was to define distinct populations within the stromal vascular fraction based on surface marker expression, and to evaluate the ability of each cell type to differentiate to mature adipocytes. Subcutaneous whole adipose tissue was obtained by abdominoplasty from human patients. The stromal vascular fraction was separated and four cell populations were isolated by flow cytometry and studied. Candidate perivascular cells (pericytes) were defined as CD146+/CD31−/CD34−. Two CD31+ endothelial populations were detected and differentiated by CD34 expression. These were tentatively designated as mature endothelial (CD 31+/CD34−), and immature endothelial (CD31+/CD34+). Both endothelial populations were heterogeneous with respect to CD146. The CD31−/CD34+ fraction (preadipocyte candidate) was also CD90+ but lacked CD146 expression. Proliferation was greatest in the CD31−/CD34+ group and slowest in the CD146+ group. Expression of adipogenic genes, peroxisome proliferator-activated receptor-γ, and fatty acid binding protein 4, were significantly higher in the CD31−/CD34+ group compared with all other populations after in vitro adipogenic differentiation. This group also demonstrated the highest proportion of AdipoRed lipid staining. The authors have isolated four distinct stromal populations from human adult adipose tissue and characterized their adipogenic potential. Of these four populations, the CD31/CD34+ group is the most prevalent and has the greatest potential for adipogenic differentiation. This cell type appears to hold the most promise for adipose tissue engineering.