Sensitive and selective Affimer-functionalised interdigitated electrode-based capacitive biosensor for Her4 protein tumour biomarker detection.
Sensitive and selective Affimer-functionalised interdigitated electrode-based capacitive biosensor for Her4 protein tumour biomarker detection.
复制标题
DOI：
10.1016/j.bios.2018.02.041
复制
发表时间：
2018-06
影响因子：
12.6
通讯作者：
Pavel Zhurauski;S. Arya;Pawan Jolly;C. Tiede;D. Tomlinson;P. Ko Ferrigno;P. Estrela
中科院分区：
文献类型：
--
作者：
Pavel Zhurauski;S. Arya;Pawan Jolly;C. Tiede;D. Tomlinson;P. Ko Ferrigno;P. Estrela
开发了一种新型的基于亲和功能化交叉指状电极的电容式生物传感器平台，用于检测和评估未稀释血清中的蛋白质肿瘤生物标志物HER4。利用带有半胱氨酸C端的抗HER4分子在金交叉电极上自组装形成生物识别层，用于传感器的制作。在没有氧化还原标志物的情况下，使用电化学阻抗谱(EIS)通过监测电容的变化来评估传感器的性能。该传感器在缓冲液和未稀释血清中具有较高的灵敏度，动态范围为1 PM~10 0 nm，在缓冲液和血清中的检测下限均小于1 PM。此外，亲和分子传感器表现出极好的特异性，几乎不受血清蛋白的干扰，这表明该传感器对非特异性结合具有弹性。目前的仿射分子传感器在添加未稀释的血清中的传感能力表明，它有可能成为一种新的基于仿射分子的传感器。因此，所制造的仿射传感器可以进一步与具有与其他生物标记物的亲和力的其他探针相适应，用于新的生物传感器范围。
A novel Affimer-functionalised interdigitated electrode-based capacitive biosensor platform was developed for detection and estimation of Her4, a protein tumour biomarker, in undiluted serum. An anti-Her4 Affimer with a C-terminal cysteine was used to create the bio-recognition layer via self-assembly on gold interdigitated electrodes for the sensor fabrication. Electrochemical impedance spectroscopy (EIS) in the absence of redox markers was used to evaluate the sensor performance by monitoring the changes in capacitance. The Affimer sensor in buffer and in undiluted serum demonstrated high sensitivity with a broad dynamic range from 1 pM to 100 nM and a limit of detection lower than 1 pM both in buffer and in serum. Furthermore, the Affimer sensor demonstrated excellent specificity with negligible interference from serum proteins, suggesting resilience to non-specific binding. The sensing ability of the present Affimer sensor in spiked undiluted serum suggests its potential for a new range of Affimer-based sensors. The fabricated Affimer sensor can thus be further adapted with other probes having affinities to other biomarkers for a new range of biosensors.