Influence of risk factors on renal leptospiral load in naturally infected wild black rats
Influence of risk factors on renal leptospiral load in naturally infected wild black rats
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DOI：
10.1016/j.actatropica.2012.11.011
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发表时间：
2013-03-01
期刊：
影响因子：
2.7
通讯作者：
Chiroleu, F.
中科院分区：
文献类型：
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作者：
Desvars, A.;Michault, A.;Chiroleu, F.
2009年，在留尼汪岛进行了一项调查，以确定被困在野外的黑鼠的肾钩端螺旋体负荷。使用qPCR计算肾组织中钩端螺旋体的浓度。我们的研究结果显示，在自然感染的黑鼠中，肾脏细菌负荷的个体间差异很大（平均值= 8.27 +/- 4.72 log基因组拷贝/mg肾脏组织）。本研究的目的是在50只自然感染的黑鼠中建立肾钩端螺旋体负荷模型，作为性别、年龄和体重的函数。按性别进行的统计分析显示，在自然感染的男性中，肾钩端螺旋体负荷与体重相关（p值= 0.032）。此外，我们的模型表明，体重和性别是显着的解释变量的肾钩端螺旋体负荷在自然感染的年轻的黑大鼠（R-2 = 0.953）。实验室实验无法复制自然获得的感染，但实地研究也存在许多局限性。我们的研究是第一次试图解释自然感染的水库动物肾钩端螺旋体负荷的个体差异，但需要进一步的研究。(c)2012爱思唯尔有限公司版权所有。
In 2009, a survey was conducted in Reunion Island to determine the renal leptospiral load in black rats trapped in the field. The concentration of leptospires in kidney tissue was calculated using qPCR. Our results showed high inter-individual variations of renal bacterial load in naturally infected black rats (mean = 8.27 +/- 4.72 log-genome copies per mg kidney tissue). The objective of this study was to model the renal leptospiral load in 50 naturally infected black rats as a function of sex, age, and weight. Statistical analysis by sex showed that, in naturally infected males, the renal leptospiral load was correlated with weight (p-value = 0.032). Moreover, our model showed that weight and sex were significant explanatory variables for the renal leptospiral load in naturally infected young black rats (R-2 = 0.953). Laboratory experimentation could not replicate naturally acquired infection, but field studies also present many limitations. Our study is the first attempt to explain individual variations in the renal leptospiral load in naturally infected reservoir animals but further research is needed. (c) 2012 Elsevier B.V. All rights reserved.