Excretion of unchanged volatile organic compounds (toluene, ethylbenzene, xylene and mesitylene) in urine as result of experimental human volunteer exposure
Excretion of unchanged volatile organic compounds (toluene, ethylbenzene, xylene and mesitylene) in urine as result of experimental human volunteer exposure
复制标题
DOI：
10.1007/s00420-007-0233-9
复制
发表时间：
2008-02-01
影响因子：
3
通讯作者：
Jalowiecki, Piotr
中科院分区：
文献类型：
--
作者：
Janasik, Beata;Jakubowski, Marek;Jalowiecki, Piotr
目的研究甲苯(TOL)、乙苯(EB)、二甲苯(XYL)和均三甲苯(MES)在受控条件下的尿中挥发性有机物(VOC)排出情况，并利用各自的数据进行职业暴露评估。方法采用顶空气相色谱和固相微萃取技术对接触过程中和接触后的血、尿中VOC的代谢产物进行分析。结果VOC在尿中的消除符合开放的二室模型。半衰期T-1/2分别为0.45~0.88h和6.7~19.2h。该方法测定不变VOC的精密度与血中不变化合物相近，优于尿液中主要代谢物。结论即使在空气中VOC含量远低于现行职业接触TWA标准的范围内，测定尿中不变VOC也可作为暴露试验。
Objectives To investigate elimination of unchanged volatile organic compounds (VOC's) through urine and the use of respective data for occupational exposure assessment, six volunteers were exposed under controlled conditions to toluene (TOL), ethylbenzene (EB), xylene (XYL) and mesitylene (MES) at concentrations ranging from 20 to 200 mg/m(3). The study was to elicit the toxicokinetic data and compare the precision of VOC's exposure assessment based on determining unchanged compounds in blood, urine and their metabolites in urine.Methods During and after exposure blood and urine samples were analysed by gas chromatography using the headspace and SPME headspace techniqueResults The kinetics of VOC's elimination in urine complied with an open two-compartment model. The (half-time) T-1/2 values varied from 0.45 to 0.88 h for phase I and from 6.7 to 19.2 h for phase II. The precision of the method for unchanged VOC's was similar to that based on unchanged compounds in blood and better than their main metabolites in urine.Conclusions The obtained result indicate that determining unchanged VOC's in urine can be used as an exposure test even in the ranges of VOC's in the air that are much lower than the current TWA for occupational exposure.