Osteoclast stimulatory transmembrane protein induces a phenotypic switch in macrophage polarization suppressing an M1 pro-inflammatory state
Osteoclast stimulatory transmembrane protein induces a phenotypic switch in macrophage polarization suppressing an M1 pro-inflammatory state
复制标题
破骨细胞刺激跨膜蛋白诱导巨噬细胞极化表型转换，抑制 M1 促炎状态
DOI：
10.1093/abbs/gmx092
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发表时间：
2017-10-01
影响因子：
3.7
通讯作者：
Chen, Fengling
中科院分区：
文献类型：
--
作者：
Yuan, Huimin;He, Jiangping;Chen, Fengling
巨噬细胞是代谢综合征的关键细胞，也是代谢性疾病的危险因素。巨噬细胞具有不同的功能和转录谱，但都是维持体内平衡所必需的。众所周知，巨噬细胞在炎症和早期动脉粥样硬化形成中起关键作用，并且存在两种表型：促炎（M1）和抗炎（M2）。破骨细胞刺激跨膜蛋白（oc-stamp）是一种多通道跨膜蛋白，但其功能尚不清楚。在这项研究中，我们探讨了oc-stamp在巨噬细胞生理学中的作用。结果表明，LPS和IFN-γ刺激后，α-stamp明显降低，IL-4刺激后，α-stamp明显升高。此外，oc-stamp诱导巨噬细胞极化的表型转换，抑制过表达组中的M1促炎状态，并促进敲除组中的M1促炎状态。进一步的研究表明，α-stamp可能通过STAT 6调节巨噬细胞的极化。综上所述，我们的结果首次证明了α-stamp可能在巨噬细胞极化中发挥重要作用并抑制M1促炎状态。
Macrophages are the key cells in metabolic syndrome and are also a risk factor for metabolic disease. Macrophages have different functions and transcriptional profiles, but all are required for maintaining homeostasis. It is well known that macrophages play a key role in inflammation and early atherogenesis, and are present in two phenotypes: pro-inflammatory (M1) and anti-inflammatory (M2). Osteoclast stimulatory transmembrane protein (oc-stamp) is a multiple-pass transmembrane protein; however, its function remains unclear. In this study, we explored the role of oc-stamp in macrophages physiology. The results showed that oc-stamp was notably decreased under LPS and IFN-γ stimulation, while it was increased with IL-4 treatment. Furthermore, oc-stamp induced a phenotypic switch in macrophage polarization, suppressing the M1 pro-inflammatory state in the overexpression group, and promoting the M1 pro-inflammatory state in the knockdown group. Further study revealed that oc-stamp regulated macrophage polarization possibly via STAT6. Taken together, our results are the first to demonstrate that oc-stamp may play an important role in macrophage polarization and inhibit the M1 pro-inflammatory state.