Low-energy shock waves evoke intracellular Ca2+ increases independently of sonoporation
Low-energy shock waves evoke intracellular Ca2+ increases independently of sonoporation
复制标题
低能量冲击波引起细胞内 Ca2+ 的增加，与声孔无关
DOI：
10.1038/s41598-019-39806-x
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发表时间：
2019
影响因子：
4.6
通讯作者：
Akira Tsukamoto
中科院分区：
文献类型：
--
作者：
Toru Takahashi;Keiichi Nakagawa;Shigeru Tada;Akira Tsukamoto
低能量冲击波(LESWs)可加速多种组织损伤的愈合，包括血管生成和骨折。在细胞中，LESW辐射增强了基因表达和蛋白质合成。这种增强背后的一个可能机制是机械传感。冲击波也可以诱导声学作用。因此，音调是另一种可能的增强机制。目前还不清楚LESWs是否需要声学操作来唤起细胞反应。LESW光照射内皮细胞可引起细胞内钙离子浓度升高。充分唤醒所需的最低声能为1.7mJ/ μ。在相同的声能下，没有观察到钙黄绿素和碘化丙啶被渗透的声波作用。研究发现，LESW辐射引起的细胞内钙离子升高不需要声学处理。细胞内钙的升高与肌动蛋白细胞骨架和牵张激活的钙通道有关，但与微管无关。此外，随着钙离子通过钙通道内流，通过PLC-IP3-IP3R级联途径释放的钙离子有助于细胞内钙离子的增加。这些结果表明，LESW辐射可以独立于声波作用而引起细胞反应。相反，LESW辐射通过机械感应激发细胞反应。
Low-energy shock waves (LESWs) accelerate the healing of a broad range of tissue injuries, including angiogenesis and bone fractures. In cells, LESW irradiations enhance gene expression and protein synthesis. One probable mechanism underlying the enhancements is mechanosensing. Shock waves also can induce sonoporation. Thus, sonoporation is another probable mechanism underlying the enhancements. It remains elusive whether LESWs require sonoporation to evoke cellular responses. An intracellular Ca2+increase was evoked with LESW irradiations in endothelial cells. The minimum acoustic energy required for sufficient evocation was 1.7 μJ/mm2. With the same acoustic energy, sonoporation, by which calcein and propidium iodide would become permeated, was not observed. It was found that intracellular Ca2+increases evoked by LESW irradiations do not require sonoporation. In the intracellular Ca2+increase, actin cytoskeletons and stretch-activated Ca2+channels were involved; however, microtubules were not. In addition, with Ca2+influx through the Ca2+channels, the Ca2+release through the PLC-IP3-IP3R cascade contributed to the intracellular Ca2+increase. These results demonstrate that LESW irradiations can evoke cellular responses independently of sonoporation. Rather, LESW irradiations evoke cellular responses through mechanosensing.