INFLUENZA-VIRUS GENOME CONSISTS OF 8 DISTINCT RNA SPECIES
INFLUENZA-VIRUS GENOME CONSISTS OF 8 DISTINCT RNA SPECIES
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DOI：
10.1073/pnas.73.9.3045
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发表时间：
1976-01-01
影响因子：
11.1
通讯作者：
NEWTON, C
中科院分区：
文献类型：
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作者：
MCGEOCH, D;FELLNER, P;NEWTON, C
禽流感病毒A，禽鼠疫，基因组RNA，通过聚丙烯酰胺琼脂糖凝胶电泳含有6 M尿素，分为8个物种。分离的32 P标记的RNA种类，其特征在于消化与RNase T1和分馏所得的寡核苷酸通过2-维凝胶电泳，这表明，每个物种有一个独特的核苷酸序列。对每个基因组RNA种类与其编码的病毒蛋白进行了初步的相关性。
The genomic RNA of the avian influenza A virus, fowl plague, was fractionated into 8 species by electrophoresis in polyacrylamide-agarose gels containing 6 M urea. The separated 32P-labeled RNA species were characterized by digestion with RNase T1 and fractionation of the resulting oligonucleotides by 2-dimensional gel electrophoresis; this demonstrated that each species has a distinct nucleotide sequence. A tentative correlation of each genome RNA species with the virus protein that it encodes was made.