THE PROKARYOTIC ENHANCER BINDING-PROTEIN NTRC HAS AN ATPASE ACTIVITY WHICH IS PHOSPHORYLATION AND DNA-DEPENDENT
THE PROKARYOTIC ENHANCER BINDING-PROTEIN NTRC HAS AN ATPASE ACTIVITY WHICH IS PHOSPHORYLATION AND DNA-DEPENDENT
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DOI：
10.1002/j.1460-2075.1992.tb05281.x
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发表时间：
1992-06-01
期刊：
影响因子：
11.4
通讯作者：
DIXON, R
中科院分区：
文献类型：
--
作者：
AUSTIN, S;DIXON, R
原核激活蛋白 NTRC 与增强子样元件结合，并通过 sigma-54 RNA 聚合酶全酶催化开放复合物形成，响应氮限制激活转录。开放复合物的形成需要 NTRC 的磷酸化形式，并且该反应依赖于 ATP。我们发现 NTRC 具有 ATP 酶活性，该活性通过磷酸化被激活，并受到包含特定 NTRC 结合位点的 DNA 的存在的强烈刺激。
The prokaryotic activator protein NTRC binds to enhancer-like elements and activates transcription in response to nitrogen limitation by catalysing open complex formation by sigma-54 RNA polymerase holoenzyme. Formation of open complexes requires the phosphorylated form of NTRC and the reaction is ATP dependent. We find that NTRC has an ATPase activity which is activated by phosphorylation and is strongly stimulated by the presence of DNA containing specific NTRC binding sites.