Isolation and expression analysis of an MAPKK gene from Fenneropenaeus chinensis in response to white spot syndrome virus infection
Isolation and expression analysis of an MAPKK gene from Fenneropenaeus chinensis in response to white spot syndrome virus infection
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中国明对虾 MAPKK 基因针对白斑综合征病毒感染的分离和表达分析
DOI：
10.1016/j.fsi.2016.05.004
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发表时间：
2016-08-01
影响因子：
4.7
通讯作者：
Liu, Ning
中科院分区：
文献类型：
--
作者：
Li, Xupeng;Kong, Jie;Liu, Ning
丝裂原激活的激酶(MAPKK)是参与宿主-病毒相互作用过程的重要基因。为了更好地了解MAPKK在中国对虾与对虾白斑综合征病毒(WSSV)相互作用过程中的作用，我们从中国对虾F.研究了FcMAPKK对WSSV感染的影响。结果表明，FcMAPICK基因包含一个1227bp的开放阅读框，编码一个高度保守的蛋白，具有丝氨酸/苏氨酸蛋白激酶催化(S_TKC)结构域。FcMAPKIC的氨基酸序列与脊椎动物、无脊椎动物、植物和真菌的MAPKK同源性在11.9%~92.6%之间。FcMAPKK在正常中国对虾所有受检组织中均有表达。FcMAPKK在肝胰腺中的表达水平最高，约为鳃的2.6倍，在肌肉中最低，约为肝胰腺的0.3倍。在WSSV攻击后，肌肉、鳃和肝胰腺中FcMAPKK的表达水平都发生了变化。感染WSSV后48h，FcMAPKK在中国对虾肌肉中的表达显著上调(P<；0.01)。感染后48h，WSSV在正常中国对虾体内开始快速复制，而U0126处理组中国对虾的WSSV复制受到显著抑制(P<；0.05)。结果提示，FcMAPKK可能参与了WSSV的感染过程，在中国对虾体内复制WSSV可能需要劫持FcMAPKK。(C)爱思唯尔有限公司出版的2016年。
Mitogen-activated kinase kinase (MAPKK) is an important gene involved in the host-virus interaction process. To obtain a better understanding of MAPKK in the interaction process between the Chinese shrimp Fenneropenaeus chinensis and white spot syndrome virus (WSSV), we cloned the sequence of an MAPKK cDNA fromF. chinensis (FcMAPKK) and investigated the effect of FcMAPKK on WSSV infection. The results showed that the FcMAPICK gene contained a 1227 bp open reading frame (ORF), which encoded a highly conserved protein with a serine/threonine protein kinase catalytic (S_TKc) domain. The deduced amino acid sequence of FcMAPKIC shared identities between 11.9 and 92.6% with MAPKKs from vertebrate, invertebrate, plant and fungus species. The FcMAPKK was expressed in all the examined tissues in the normal F. chinensis. FcMAPKK expression level was highest in the hepatopancreas where it was approximately 2.6-fold the expression level in the gill, and lowest in the muscle where it was approximately 0.3-fold the expression level in the hepatopancreas. The FcMAPKK expression levels in the muscle, gill, and hepatopancreas were all changed post WSSV challenge. The FcMAPKK expression was significantly (P < 0.01) up-regulated in the muscle of F. chinensis at 48 h post WSSV infection. The WSSV began to replicate quickly in the normal F. chinensis at 48 h post infection, while the WSSV replication in the U0126-treated F. chinensis could be significantly (P < 0.05) inhibited. The results suggested that FcMAPKK might be involved in the WSSV infection process, and hijacking of FcMAPKK might be required for WSSV replication in F. chinensis. (C) 2016 Published by Elsevier Ltd.